Mohebbati Reza, Kamkar-Del Yasamin, Shafei Mohammad N
Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Applied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Endocr Metab Immune Disord Drug Targets. 2021;21(9):1620-1626. doi: 10.2174/1871530320666201104114609.
Our previous studies showed the antihypertensive effect of Ribes khorassanicum (R. khorassanicum), a medicinal herb growing in the North Khorasan Province of Iran. For further evaluation, the present study investigated the effect of n-hexane (HX), ethyl acetate (EA), and aqueous (AQ) fractions of hydroalcoholic R. khorassanicum extract on cardiovascular responses in angiotensin II (AngII) and NG-nitro-L-arginine methyl ester (L-NAME) hypertensive rats.
Wistar rats were randomly divided into 11 groups (n=5): 1) control, 2) AngII (50 ng/kg, i.v), 3) AngII + losartan (Los, 10 mg/kg, i.p), 4) L-NAME (10 mg/kg, i.v), 5) L-NAME+ sodium nitroprusside (SNP) (50 mg/kg, i.p), 6, 7, 8) one dose of each fraction of R. khorassanicum (AQ/EA/HX (50 mg/kg, i.p)) +AngII, Los 9, 10, 11) one dose of each fraction of R. khorassanicum (AQ/EA/HX (50 mg/kg, i.p)) + L-NAME. Treated rats received three fractions 30 min before the injection of L-NAME and AngII in separate groups. The cardiovascular parameters were recorded by the Power Lab instrument via an angiocath inserted into the femoral artery. The peak changes (Δ) of Mean Arterial Pressure (MAP), Systolic Blood Pressure (SBP), and Heart Rate (HR) in treated groups were compared with those of the hypertensive and control groups.
AngII and L-NAME significantly increased ΔMAP and ΔSBP and attenuated by pretreatment of Los and SNP, respectively. Pretreatment with polar (AQ) and semipolar (EA) fractions of R. khorassanicum reduced the peak changes of MAP and SBP in both AngII and L-NAME-treated groups. Only the fraction of the herb attenuated the HR increased in the L-NAME group. The HR in other groups did not demonstrate any significant difference.
All fractions of R. khorassanicum have an antihypertensive effect. However, the effect of polar fractions is more salient. It is also conceivable that the antihypertensive effect of fractions is mostly mediated by the inhibition of AngII.
我们之前的研究表明，生长在伊朗呼罗珊省北部的药用植物霍拉桑醋栗（Ribes khorassanicum）具有降压作用。为了进一步评估，本研究调查了霍拉桑醋栗水醇提取物的正己烷（HX）、乙酸乙酯（EA）和水相（AQ）部分对血管紧张素II（AngII）和N-硝基-L-精氨酸甲酯（L-NAME）高血压大鼠心血管反应的影响。
将Wistar大鼠随机分为11组（n = 5）：1）对照组，2）AngII（50 ng/kg，静脉注射），3）AngII + 氯沙坦（Los，10 mg/kg，腹腔注射），4）L-NAME（10 mg/kg，静脉注射），5）L-NAME + 硝普钠（SNP）（50 mg/kg，腹腔注射），6、7、8）霍拉桑醋栗各部分一剂（AQ/EA/HX（50 mg/kg，腹腔注射））+ AngII，Los 9、10、11）霍拉桑醋栗各部分一剂（AQ/EA/HX（50 mg/kg，腹腔注射））+ L-NAME。在单独的组中，处理后的大鼠在注射L-NAME和AngII前30分钟接受三个部分。通过插入股动脉的血管导管，使用Power Lab仪器记录心血管参数。将处理组的平均动脉压（MAP）、收缩压（SBP）和心率（HR）的峰值变化（Δ）与高血压组和对照组进行比较。
AngII和L-NAME分别显著增加ΔMAP和ΔSBP，并分别通过氯沙坦和硝普钠预处理而减弱。用霍拉桑醋栗的极性（AQ）和半极性（EA）部分预处理可降低AngII和L-NAME处理组中MAP和SBP的峰值变化。只有该草药部分减弱了L-NAME组中增加的HR。其他组的HR没有显示出任何显著差异。
霍拉桑醋栗的所有部分都有降压作用。然而，极性部分的作用更显著。也可以想象，各部分的降压作用主要是通过抑制AngII介导的。
