黑种草的标准化提取物及其主要成分百里醌可改善大鼠体内血管紧张素II诱导的高血压。
The standardized extract of Nigella sativa and its major ingredient, thymoquinone, ameliorates angiotensin II-induced hypertension in rats.
作者信息
Enayatfard Lili, Mohebbati Reza, Niazmand Saeed, Hosseini Mahmoud, Shafei Mohammad Naser
机构信息
Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Neurogenic Inflammation Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
出版信息
J Basic Clin Physiol Pharmacol. 2018 Dec 19;30(1):51-58. doi: 10.1515/jbcpp-2018-0074.
Background This study investigated the effect of hydroalcoholic extract of Nigella sativa (N. sativa) and its active component, thymoquinone (TQ) on hypertension induced by angiotensin II (AngII), the main product of renin-angiotensin system (RAS). Methods Seven animal groups (n=7 for each group) were used as follows: (1) control, (2) AngII (300 ng/kg), (3) AngII+losartan (Los; 10 mg/kg), (4) TQ (40 mg/kg)+AngII, and (5-7) three doses of N. sativa (200, 400, and 600 mg/kg)+AngII. Los and AngII were injected intravenously; TQ and extracts were injected intraperitoneally. In TQ and N. sativa-treated groups, 30 min after injection of the extract and TQ, AngII was injected. Cardiovascular parameters were recorded by power lab system after cannulation of femoral artery. The maximum changes (∆) of systolic blood pressure (SBP), mean arterial pressure (MAP), and heart rate (HR) were calculated and used for statistical analysis. Results AngII significantly increased maximal ∆SBP, ∆MAP, and ∆HR compared with the control (p<0.001), and these effects significantly were blunted by Los. TQ and two higher doses (400 and 600 mg/kg) of N. sativa significantly could antagonize effect of AngII on ∆SBP, ∆MAP (p<0.05 to p<0.001). AngII-induced changes of HR are also significantly decreased by TQ and dose 600 mg/kg of extract (p<0.01 and p<0.05, respectively). Conclusions The N. sativa and its component TQ have the beneficial effect on hypertension probably due to attenuation cardiovascular effects of AngII.
背景 本研究调查了黑种草水醇提取物及其活性成分百里醌（TQ）对肾素-血管紧张素系统（RAS）主要产物血管紧张素II（AngII）诱导的高血压的影响。方法 使用七组动物（每组n = 7），如下所示：（1）对照组，（2）AngII（300 ng/kg），（3）AngII + 氯沙坦（Los；10 mg/kg），（4）TQ（40 mg/kg）+ AngII，以及（5 - 7）黑种草的三个剂量（200、400和600 mg/kg）+ AngII。Los和AngII静脉注射；TQ和提取物腹腔注射。在TQ和黑种草治疗组中，注射提取物和TQ 30分钟后注射AngII。股动脉插管后通过功率实验室系统记录心血管参数。计算收缩压（SBP）、平均动脉压（MAP）和心率（HR）的最大变化（∆）并用于统计分析。结果 与对照组相比，AngII显著增加了最大∆SBP、∆MAP和∆HR（p < 0.001），而Los显著减弱了这些作用。TQ和黑种草的两个较高剂量（400和600 mg/kg）可显著拮抗AngII对∆SBP、∆MAP的作用（p < 0.05至p < 0.001）。TQ和600 mg/kg剂量的提取物也显著降低了AngII引起的HR变化（分别为p < 0.01和p < 0.05）。结论 黑种草及其成分TQ对高血压具有有益作用，可能是由于减弱了AngII的心血管效应。
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