Liang Changyan, Ling You, Wei Feng, Huang Lijie, Li Xiaomao
Department of Gynecology and Obstetrics, Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong, China.
National Engineering Laboratory for Regenerative Medical Implant Devices, Guanhao Biotech Group, Guangzhou Juming Biotech Co., Ltd, Guangzhou, Guangdong, China.
Regen Biomater. 2020 Sep 2;7(5):483-490. doi: 10.1093/rb/rbaa034. eCollection 2020 Oct.
The biomaterials composed of mammalian extracellular matrix (ECM) have a great potential in pelvic floor tissue repair and functional reconstruction. However, bacterial infection does cause great damage to the repair function of biomaterials which is the major problem in clinical utilization. Therefore, the development of biological materials with antimicrobial effect is of great clinical significance for pelvic floor repair. Chitosan/tigecycline (CS/TGC) antibacterial biofilm was prepared by coating CS/TGC nanoparticles on mammalian-derived ECM. Infrared spectroscopy, scanning electron microscopy, bacteriostasis circle assay and static dialysis methods were used to characterize the membrane. MTS assay kit and DAPI fluorescence staining were used to evaluate cytotoxicity and cell adhesion. The biocompatibility was assessed by subabdominal implantation model in goats. Subcutaneous antimicrobial test in rabbit back was used to evaluate the antimicrobial and repairing effects on the infected wounds . Infrared spectroscopy showed that the composite coating had been successfully modified. The antibacterial membrane retained the main structure of ECM multilayer fibers. release of biomaterials showed sustained release and stability. studies showed that the antibacterial biological membrane had low cytotoxicity, fast degradation, good compatibility, anti-infection and excellent repair ability.
由哺乳动物细胞外基质（ECM）组成的生物材料在盆底组织修复和功能重建方面具有巨大潜力。然而，细菌感染确实会对生物材料的修复功能造成极大损害，这是临床应用中的主要问题。因此，开发具有抗菌作用的生物材料对盆底修复具有重要的临床意义。通过将壳聚糖/替加环素（CS/TGC）纳米颗粒包覆在哺乳动物来源的ECM上制备了抗菌生物膜。采用红外光谱、扫描电子显微镜、抑菌圈试验和静态透析法对该膜进行表征。使用MTS检测试剂盒和DAPI荧光染色评估细胞毒性和细胞黏附情况。通过山羊腹下植入模型评估生物相容性。采用兔背部皮下抗菌试验评估对感染伤口的抗菌和修复效果。红外光谱表明复合涂层已成功改性。抗菌膜保留了ECM多层纤维的主要结构。生物材料的释放呈现缓释和稳定性。研究表明，抗菌生物膜具有低细胞毒性、快速降解、良好的相容性、抗感染能力和出色的修复能力。
