Dhanalakshmi V, Nimal T R, Sabitha M, Biswas Raja, Jayakumar R
Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham University, Kochi, 682041, India.
Amrita School of Pharmacy, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham University, Kochi, 682041, India.
J Biomed Mater Res B Appl Biomater. 2016 May;104(4):797-807. doi: 10.1002/jbm.b.33635. Epub 2016 Feb 21.
Majority of the chronic wounds are infected with bacteria like Staphylococcus aureus (S. aureus). The deep tissue infections are difficult to treat using topical antibiotics, due to their poor tissue penetration. In order to treat S. aureus deep tissue infections we have developed an antibiotic delivery system using chitosan nanoparticles (CNPs). To enhance their tissue penetration these CNPs were further coated using lecithin (CLNPs). Antibiotic tigecycline was loaded into chitosan nanoparticles (tCNPs) and then coated with lecithin to generate lecithin coated tigecycline loaded chitosan nanoparticles (tCLNPs). The prepared nanoparticles were characterized using DLS, SEM, TEM and FT-IR. The prepared CNPs, tCNPs, CLNPs and tCLNPs have the size range of 85 ± 10, 90 ± 18, 188 ± 5 and 235 ± 20 nm, respectively. The tCLNPs shows more sustained release pattern of tigecycline. The antibacterial activity of the developed nanoparticles was confirmed against laboratory and clinical strains of S. aureus using in vitro and ex vivo experiments. The ex vivo skin and muscle permeation study ensures the enhanced delivery of tigecycline to the deeper tissue. The prepared nanoparticles were hemo-compatible and cyto-compatible. Our study suggests that the prepared tCLNPs can be effectively used for the treatment of S. aureus infected wounds.
大多数慢性伤口感染了金黄色葡萄球菌等细菌。由于局部抗生素的组织穿透性较差，深部组织感染难以用其进行治疗。为了治疗金黄色葡萄球菌深部组织感染，我们开发了一种使用壳聚糖纳米颗粒（CNP）的抗生素递送系统。为了增强其组织穿透性，这些CNP进一步用卵磷脂包被（CLNP）。将抗生素替加环素负载到壳聚糖纳米颗粒（tCNP）中，然后用卵磷脂包被，以生成卵磷脂包被的负载替加环素的壳聚糖纳米颗粒（tCLNP）。使用动态光散射（DLS）、扫描电子显微镜（SEM）、透射电子显微镜（TEM）和傅里叶变换红外光谱（FT-IR）对制备的纳米颗粒进行表征。制备的CNP、tCNP、CLNP和tCLNP的粒径范围分别为85±10、90±18、188±5和235±20nm。tCLNP显示出替加环素更持续的释放模式。使用体外和离体实验证实了所开发的纳米颗粒对金黄色葡萄球菌实验室菌株和临床菌株的抗菌活性。离体皮肤和肌肉渗透研究确保了替加环素向更深组织的增强递送。制备的纳米颗粒具有血液相容性和细胞相容性。我们的研究表明，所制备的tCLNP可有效地用于治疗金黄色葡萄球菌感染的伤口。
