Department of Chemistry, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.
Chem Commun (Camb). 2020 Dec 4;56(93):14519-14540. doi: 10.1039/d0cc05450j. Epub 2020 Nov 5.
Artificial metalloenzymes have been recently established as efficient alternatives to traditional transition metal catalysts. The presence of a secondary coordination sphere in artificial metalloenzymes makes them advantageous over transition metal catalysts, which rely essentially on their first coordination sphere to exhibit their catalytic activity. Recent developments on streptavidin- and avidin-based artificial metalloenzymes have made them highly chemically and genetically evolved for selective organometallic transformations. In this review, we discuss the chemo-genetic optimization of streptavidin- and avidin-based artificial metalloenzymes for the enhancement of their catalytic activities towards a wide range of synthetic transformations. Considering the high impact in vivo applications of artificial metalloenzymes, their catalytic efficacies to promote abiological reactions in intracellular as well as periplasmic environment are also discussed. Overall, this review can provide an insight to readers regarding the design and systematic optimization of strept(avidin)-based artificial metalloenzymes for specific reactions.
人工金属酶最近被确立为传统过渡金属催化剂的有效替代品。人工金属酶中存在次级配位球，这使它们比过渡金属催化剂更具优势，后者主要依赖其第一配位球来表现其催化活性。基于链霉亲和素和亲和素的人工金属酶的最新进展使它们在选择性有机金属转化方面具有高度的化学和遗传进化。在这篇综述中，我们讨论了链霉亲和素和亲和素基人工金属酶的化学生物优化，以提高它们对广泛的合成转化的催化活性。考虑到人工金属酶在体内的高影响应用，我们还讨论了它们在细胞内和周质环境中促进非生物反应的催化效率。总的来说，这篇综述可以为读者提供有关针对特定反应设计和系统优化基于链霉（亲和素）的人工金属酶的见解。
