Oral and Dental Disease Research Center, Department of Pediatric Dentistry, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.
Oral and Dental Disease Research Center, Department of Dental Public Health, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.
PLoS One. 2020 Nov 5;15(11):e0241272. doi: 10.1371/journal.pone.0241272. eCollection 2020.
To assess- the release of calcium and phosphate ions from a fissure sealant containing amorphous calcium phosphate (ACP), and to determine the re-release capacity of these ions when charged with a solution containing casein phosphopeptide-amorphous calcium phosphate (CPP-ACP). Nine blocks of ACP resin-based sealant were prepared and immersed in three solutions at different pH (4.0, 5.5, 7.0), and calcium and phosphate ion release was measured with ion chromatography at 1, 3, 5, 7, 14, 21 and 28 days after immersion. Sixty days after immersion, each block was charged with CPP-ACP solution in three 7-day cycles to investigate the re-release of these ions, which was measured on days 1, 3, and 7. No difference was observed in initial calcium ion release at pH 4.0 and pH 5.5. At both values, ion release was significantly higher than at pH 7.0 (p<0.001). Initial phosphate release was significantly different among the three pH values (p<0.001). After re-charging the specimens, calcium ion re-release was greater than phosphate ion release. Initial ion release from ACP resin-based sealant was greatest at the lowest pH. Ion release decreased with time. As the number of recharge cycles increased, ion re-release also improved. Phosphate ion re-release required more recharge cycles than calcium ion re-release.
评估含有无定形磷酸钙（ACP）的裂缝密封剂中钙离子和磷酸根离子的释放，并确定这些离子在被含有酪蛋白磷酸肽-无定形磷酸钙（CPP-ACP）的溶液充电时的再释放能力。制备了 9 个 ACP 树脂基密封剂块，并将其浸入三种不同 pH 值（4.0、5.5、7.0）的溶液中，在浸入后 1、3、5、7、14、21 和 28 天用离子色谱法测量钙离子和磷酸根离子的释放。浸入 60 天后，每个块用 CPP-ACP 溶液进行三个 7 天的充电循环，以研究这些离子的再释放情况，在第 1、3 和 7 天进行测量。在 pH 值为 4.0 和 pH 值为 5.5 时，初始钙离子释放没有差异。在这两个值下，离子释放明显高于 pH 值为 7.0（p<0.001）。初始磷酸盐释放在三种 pH 值之间有显著差异（p<0.001）。在重新充电后，钙离子的再释放大于磷酸根离子的释放。ACP 树脂基密封剂的初始离子释放在最低 pH 值时最大。随着时间的推移，离子释放减少。随着充电循环次数的增加，离子的再释放也得到改善。磷酸根离子的再释放需要比钙离子的再释放更多的充电循环。
