School of Food & Biological Engineering, Institute of Food Physical Processing, Jiangsu University, Zhenjiang 212013, China.
School of Food & Biological Engineering, Institute of Food Physical Processing, Jiangsu University, Zhenjiang 212013, China.
Food Chem. 2021 Mar 1;339:128081. doi: 10.1016/j.foodchem.2020.128081. Epub 2020 Sep 16.
In the present study, three-phase partitioning (TPP) coupled with gradient ethanol precipitation (GEP) was developed for the first time to extract and isolate polysaccharides (GPSs) from raw garlic (Allium sativum L.) bulbs. Four kinds of fructose polymers, namely, GPS35, GPS50, GPS65, and GPS80, were obtained at the final ethanol precipitation concentrations of 35%, 50%, 65%, and 80% (v/v), respectively, and their physicochemical characteristics and in vitro biological activities were investigated. Results indicated that GPS80 had higher carbohydrate (86.68% ± 0.90%) and uronic acid (12.89% ± 0.09%) contents, lower weight-average molecular weight (8.93 × 10 Da), and looser surface morphology than the other three GPSs. Furthermore, among the four GPSs, GPS80 exhibited the strongest antioxidant capacities, inhibitory effects on α-amylase and α-glycosidase, and nitric oxide stimulatory activity on RAW264.7 macrophage cells in vitro. Therefore, this study provides a simple and feasible technological strategy for producing bioactive polysaccharides from raw Allium vegetables.
在本研究中，首次开发了三相分配（TPP）与梯度乙醇沉淀（GEP）相结合的方法，从生大蒜（Allium sativum L.）鳞茎中提取和分离多糖（GPSs）。在最终的乙醇沉淀浓度为 35%（v/v）、50%（v/v）、65%（v/v）和 80%（v/v）时，分别得到了四种果糖聚合物，即 GPS35、GPS50、GPS65 和 GPS80，并对它们的理化特性和体外生物活性进行了研究。结果表明，GPS80 的碳水化合物（86.68%±0.90%）和糖醛酸（12.89%±0.09%）含量较高，重均分子量（8.93×10 Da）较低，表面形态较松散。此外，在这四种 GPSs 中，GPS80 表现出最强的抗氧化能力、对α-淀粉酶和α-糖苷酶的抑制作用，以及对 RAW264.7 巨噬细胞细胞的一氧化氮刺激活性。因此，本研究为从生大蒜等蔬菜中生产具有生物活性的多糖提供了一种简单可行的技术策略。
