Kim DongGwan, Bahmani Ramin, Modareszadeh Mahsa, Hwang Seongbin
Department of Bioindustry and Bioresource Engineering, Department of Molecular Biology and Plant Engineering Research Institute, Sejong University, Seoul 05006, Korea.
Plants (Basel). 2020 Nov 3;9(11):1480. doi: 10.3390/plants9111480.
Arsenite [As(III)] is a highly toxic chemical to all organisms. Previously, we reported that the overexpression of enhanced As(III) tolerance and reduced As(III) accumulation in yeast () and tobacco (). To understand a mechanism for higher As(III) tolerance and lower As(III) accumulation in -overexpressing tobacco, we examined the expression levels of various putative As(III) transporters (aquaporin). The expressions of putative As(III) exporter , , , , , and were enhanced, while the expressions of putative As(III) importer , , and were decreased, contributing to the reduced accumulation of As(III) in -overexpressing tobacco. In addition, the levels of oxidative stress indicators (HO, superoxide and malondialdehyde) were lower, and the activities of antioxidant enzymes (catalase, superoxide dismutase and glutathione reductase) were higher in -tobacco than in the control tobacco. This suggests that the lower oxidative stress in transgenic tobacco may be attributed to the higher activities of antioxidant enzymes and lower As(III) levels. Taken together, the overexpression of enhances As(III) tolerance by reducing As(III) accumulation through modulation of expressions of putative As(III) transporters in tobacco.
亚砷酸盐[As(III)]对所有生物都是一种剧毒化学物质。此前，我们报道过[具体基因名称未给出]的过表达增强了酵母（[酵母名称未给出]）和烟草（[烟草名称未给出]）对As(III)的耐受性并减少了As(III)的积累。为了解过表达[具体基因名称未给出]的烟草对As(III)具有更高耐受性和更低积累量的机制，我们检测了各种假定的As(III)转运蛋白（水通道蛋白）的表达水平。假定的As(III)输出蛋白[具体蛋白名称未给出]、[具体蛋白名称未给出]、[具体蛋白名称未给出]、[具体蛋白名称未给出]、[具体蛋白名称未给出]和[具体蛋白名称未给出]的表达增强，而假定的As(III)输入蛋白[具体蛋白名称未给出]、[具体蛋白名称未给出]和[具体蛋白名称未给出]的表达降低，这有助于减少过表达[具体基因名称未给出]的烟草中As(III)的积累。此外，与对照烟草相比，过表达[具体基因名称未给出]的烟草中氧化应激指标（过氧化氢、超氧化物和丙二醛）水平较低，抗氧化酶（过氧化氢酶、超氧化物歧化酶和谷胱甘肽还原酶）活性较高。这表明转基因烟草中较低的氧化应激可能归因于抗氧化酶的较高活性和较低的As(III)水平。综上所述，[具体基因名称未给出]的过表达通过调节烟草中假定的As(III)转运蛋白的表达来减少As(III)积累，从而增强了对As(III)的耐受性。
