Kingwell B, Rattner J B
Department of Medical Biochemistry, University of Calgary, Alberta, Canada.
Chromosoma. 1987;95(6):403-7. doi: 10.1007/BF00333991.
The composition of the mammalian kinetochore/centromere was studied by indirect immunofluorescence and immunoblotting protocols using serum from a patient with the CREST variant of scleroderma. The results of these studies suggest that a protein with a molecular weight of 50 kDa is localized at the surface of the primary constrictions (the kinetochore region) of both human and Indian muntjac chromosomes. In addition, we were able to verify the presence of a 19.5 kDa antigen (CENP-A), previously detected in human centromeres, within the kinetochore region of the Indian muntjac. These data suggest that the composition of the kinetochore region of the primary constriction is complex and that there is conservation in composition within the mammals. These features may reflect the important role of this unique chromosomal domain in the maintenance of ploidy.
利用一名患有硬皮病CREST变异型患者的血清，通过间接免疫荧光和免疫印迹法研究了哺乳动物动粒/着丝粒的组成。这些研究结果表明，一种分子量为50 kDa的蛋白质定位于人类和印度麂染色体的主缢痕（动粒区域）表面。此外，我们能够证实先前在人类着丝粒中检测到的一种19.5 kDa抗原（CENP-A）存在于印度麂的动粒区域内。这些数据表明，主缢痕动粒区域的组成是复杂的，并且在哺乳动物中组成具有保守性。这些特征可能反映了这个独特染色体结构域在维持倍性方面的重要作用。
