筛选阻断 PD-1/PD-L1 相互作用的 threose 核酸适体用于癌症免疫治疗。
Selection of threose nucleic acid aptamers to block PD-1/PD-L1 interaction for cancer immunotherapy.
机构信息
Department of Biomedical Engineering, College of Engineering and Applied Sciences, Jiangsu Key Laboratory of Artificial Functional Materials, Nanjing University, Nanjing, Jiangsu 210023, China.
出版信息
Chem Commun (Camb). 2020 Dec 4;56(93):14653-14656. doi: 10.1039/d0cc06032a. Epub 2020 Nov 6.
Threose nucleic acid (TNA) aptamers were selected in vitro to bind PD-L1 protein and inhibit its interaction with PD-1. These biologically stable TNA aptamers bound target proteins with nanomolar affinities, and effectively blocked PD-1/PD-L1 interaction in vitro. After injection into a colon cancer xenograft mouse model, the TNA aptamer N5 was specifically accumulated at the tumour site, and significantly inhibited tumour growth in vivo.
三碳核酸（TNA）适体在体外被筛选出来以结合 PD-L1 蛋白并抑制其与 PD-1 的相互作用。这些具有生物稳定性的 TNA 适体以纳摩尔亲和力结合靶蛋白，并有效地在体外阻断 PD-1/PD-L1 相互作用。在注射到结肠癌异种移植小鼠模型后，TNA 适体 N5 特异性地在肿瘤部位积聚，并显著抑制体内肿瘤生长。
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