Department of Biomedical Engineering, College of Engineering and Applied Sciences, Jiangsu Key Laboratory of Artificial Functional Materials, Nanjing University, Nanjing, Jiangsu 210023, China.
Chem Commun (Camb). 2020 Dec 4;56(93):14653-14656. doi: 10.1039/d0cc06032a. Epub 2020 Nov 6.
Threose nucleic acid (TNA) aptamers were selected in vitro to bind PD-L1 protein and inhibit its interaction with PD-1. These biologically stable TNA aptamers bound target proteins with nanomolar affinities, and effectively blocked PD-1/PD-L1 interaction in vitro. After injection into a colon cancer xenograft mouse model, the TNA aptamer N5 was specifically accumulated at the tumour site, and significantly inhibited tumour growth in vivo.
三碳核酸（TNA）适体在体外被筛选出来以结合 PD-L1 蛋白并抑制其与 PD-1 的相互作用。这些具有生物稳定性的 TNA 适体以纳摩尔亲和力结合靶蛋白，并有效地在体外阻断 PD-1/PD-L1 相互作用。在注射到结肠癌异种移植小鼠模型后，TNA 适体 N5 特异性地在肿瘤部位积聚，并显著抑制体内肿瘤生长。
