Institute of Molecular Medicine, University of Texas Health Science Center at Houston, 1825 Pressler Street, Houston, TX, 77030, USA.
AM Biotechnologies, LLC, 12521 Gulf Freeway, Houston, TX, 77034, USA.
Biochimie. 2018 Feb;145:125-130. doi: 10.1016/j.biochi.2017.09.006. Epub 2017 Sep 11.
Specific, chemically modified aptamers (X-Aptamers) were identified against two immune checkpoint proteins, recombinant Programmed Death 1 (PD-1) and Programmed Death Ligand 1 (PD-L1). Selections were performed using a bead-based X-Aptamer (XA) library containing several different amino acid functional groups attached to dU at the 5-position. The binding affinities and specificities of the selected XA-PD1 and XA-PDL1 were validated by hPD-1 and hPD-L1 expression cells, as well as by binding to human pancreatic ductal adenocarcinoma tissue. The selected PD1 and PDL1 XAs can mimic antibody functions in in vitro assays.
针对两种免疫检查点蛋白，即重组程序性死亡受体 1（PD-1）和程序性死亡配体 1（PD-L1），鉴定了特定的、化学修饰的适体（X-适体）。选择是使用一种基于珠子的 X-适体（XA）文库进行的，该文库包含了几种不同的氨基酸官能团，连接在 dU 的 5 位上。所选 XA-PD1 和 XA-PDL1 的结合亲和力和特异性通过 hPD-1 和 hPD-L1 表达细胞以及与人胰腺导管腺癌组织的结合进行了验证。所选的 PD1 和 PDL1 XAs 可以在体外实验中模拟抗体的功能。
