Riley T V, Brazier J S, Hassan H, Williams K, Phillips K D
PHLS Anaerobe Reference Unit, Luton.
Epidemiol Infect. 1987 Oct;99(2):355-9. doi: 10.1017/s0950268800067832.
Two enrichment methods were compared for their ability to recover Clostridium difficile from stool samples. One method used selective enrichment in an antibiotic-containing broth followed by detection with a latex particle agglutination (LPA) reagent. The other used enrichment in a non-selective broth following treatment of the specimen with alcohol. With clinical specimens enrichment culture was significantly more successful at detecting C. difficile than direct plating. Alcohol shock enrichment was twice as effective as direct culture, while selective broth enrichment was three times more effective. The use of LPA for screening selective enrichment broths for C. difficile should prove a cost-effective measure as only positive broths (about 20%) require subculture for confirmation.
比较了两种从粪便样本中分离艰难梭菌的富集方法。一种方法是在含抗生素的肉汤中进行选择性富集，然后用乳胶颗粒凝集（LPA）试剂进行检测。另一种方法是在用酒精处理样本后，在非选择性肉汤中进行富集。对于临床样本，富集培养在检测艰难梭菌方面比直接平板接种显著更成功。酒精休克富集的效果是直接培养的两倍，而选择性肉汤富集则是其三倍。使用LPA筛选艰难梭菌的选择性富集肉汤应是一种具有成本效益的措施，因为只有阳性肉汤（约20%）需要传代培养以进行确认。
