Akter Nahid, Hashim Roshada, Pham Huy Quang, Choi Seung-Dae, Lee Dong-Woo, Shin Jae-Ho, Rajagopal Kammara
Department of Aquaculture, Faculty of Fisheries, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh.
School of Biological sciences, Universiti Sains Malaysia, George Town, Malaysia.
Front Microbiol. 2020 Oct 9;11:570851. doi: 10.3389/fmicb.2020.570851. eCollection 2020.
We identified an antimicrobial peptide (AMP) from that was antagonistic to . studies such as well-diffusion and field trials revealed that the AMP was active against . The field trials of AMP using -infected with a mannone oligosaccharide (MOS) prebiotic, antigens, -infected fish serum, , and cell free-supernatant (LABS-CFS) on an indicator organism further revealed that the antimicrobial agent could protect . . Other than the AMP, none of the above were able to eliminate the infectious agent , and were only able to delay the death rate for 3-4 days. Thus, we conclude that the AMP is antagonistic to and may be used for treatment of infections. Subsequent whole-genome sequence analyses enabled an understanding of the (probable) gene arrangement and its location on the chromosome. This information may be useful in the generation of recombinant peptides to produce larger quantities for treatment.
我们从……中鉴定出一种对……具有拮抗作用的抗菌肽(AMP)。诸如琼脂扩散和田间试验等研究表明,该AMP对……具有活性。使用甘露寡糖(MOS)益生元、……抗原、……感染鱼血清、……以及指示生物上的无细胞上清液(LABS - CFS)对感染……的……进行AMP的田间试验,进一步表明该抗菌剂可以保护……。除了AMP之外,上述其他物质均无法消除感染因子……,仅能将死亡率推迟3 - 4天。因此,我们得出结论,该AMP对……具有拮抗作用,可用于治疗……感染。随后的……全基因组序列分析有助于了解(可能的)基因排列及其在染色体上的位置。这些信息可能有助于生成重组肽以大量生产用于治疗。