Faculty of Resource Science and Technology, Univeriti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia.
Faculty of Science and Technology, Universiti Sains Islamic Malaysia, 71800 Bandar Baru Bangi, Malaysia.
Fish Shellfish Immunol. 2018 Apr;75:99-108. doi: 10.1016/j.fsi.2018.02.005. Epub 2018 Feb 3.
This study examined the effect of dietary prebiotics and probiotics after 16 weeks, followed by 8 weeks of post feeding trial with the control unsupplemented diet on haematological and immune response against Aeromonas hydrophila infection in Channa striata fingerlings. Fish were raised on a 40% protein and 12% lipid feed containing three commercial prebiotics (β-glucan, GOS or galacto-oligosaccharide, MOS or mannan-oligosaccharide); and two probiotics- (Saccharomyces cerevisiae, Lactobacillus acidophilus), respectively and a control. Throughout the study, supplementation with dietary prebiotics and probiotics led to significant (P < 0.05) improvement in the red blood cells, white blood cells, packed cell volume, haemoglobin concentration and serum protein level and lysozyme activities; and these improvements were effective significantly (P < 0.05) when the fish were challenged with Aeromonas hydrophila at the dose of 2 × 10. The disease resistance against A. hydrophila was higher significantly (P < 0.05) in fish fed with probiotic feed supplements (L.acidophilus was highest) compared to prebiotics and control. The study is the first to report the absence of differences in sustaining the efficacies attained after intake of β-glucan, GOS and MOS upon post-feeding with an unsupplemented feed, over a prolonged period.
本研究考察了在 16 周的时间里补充益生元和益生菌，然后在 8 周的喂养试验中用未补充的对照饮食喂养后，对卵形鲳鲹鱼苗的血液学和对嗜水气单胞菌感染的免疫反应的影响。研究人员用含有三种商业益生元（β-葡聚糖、GOS 或半乳糖寡糖、MOS 或甘露寡糖）和两种益生菌（酿酒酵母、嗜酸乳杆菌）的 40%蛋白质和 12%脂肪饲料喂养鱼，并设一个对照组。在整个研究过程中，补充益生元和益生菌显著（P < 0.05）提高了红细胞、白细胞、红细胞压积、血红蛋白浓度和血清蛋白水平以及溶菌酶活性；当用 2×10 的剂量对鱼进行嗜水气单胞菌攻毒时，这些改善效果显著（P < 0.05）。与益生元相比，益生菌饲料补充剂（嗜酸乳杆菌最高）喂养的鱼对嗜水气单胞菌的抗病能力显著（P < 0.05）提高。本研究首次报道了在延长时间内，用未补充的饲料进行后喂养后，摄入β-葡聚糖、GOS 和 MOS 后获得的疗效没有差异。
