Kıvanç Merih, Er Sevda
Department of Biology, Faculty of Sciences, Eskisehir Technical University, 26470, Eskisehir, Turkey.
Pharmacy Services Program, Yunus Emre Vocational School of Health Services, Anadolu University, 26470, Eskisehir, Turkey.
Afr Health Sci. 2020 Jun;20(2):641-648. doi: 10.4314/ahs.v20i2.12.
In this study, it was aimed to investigate the effects of bacterial cells and cell-free filtrates of 8MR7 and L subspecies paracasei 10MR8 on the biofilm formation of 3 , 3 and 12 isolated from the vagina and identified their virulence factors.
Haemolytic activities esterase activities, and phospholipase activities as virulence factors of Candida strains were determined. Biofilm formations of these isolates were determined by Congo Red agar and microtitration plate method. Antibiofilm activities of bacterial cells and cell-free filtrates of 8MR7 and subspecies paracasei 10MR8 on Candida isolates were determined by the microtitration plate method.
Bacterial cells of 8MR7 and subspecies paracasei 10MR8 were not very effective in the inhibition of biofilm, whereas it has been observed that the cell-free filtrates of these bacteria inhibit the formation of biofilms of Candida strains. Although the main mechanism for inhibiting the formation of biofilm is the competition for adhesion, it is concluded that the substances contained in the cell-free filtrates of lactic acid bacteria are also important.
These isolates promise hope as potential bacteria that can be used for anti-adhesion purposes in health-care materials.
本研究旨在探讨副干酪乳杆菌8MR7和副干酪亚种10MR8的细菌细胞及无细胞滤液对从阴道分离出的3种、3种和12种念珠菌生物膜形成的影响，并鉴定其毒力因子。
测定念珠菌菌株作为毒力因子的溶血活性、酯酶活性和磷脂酶活性。通过刚果红琼脂和微量滴定板法测定这些分离株的生物膜形成。采用微量滴定板法测定8MR7和副干酪亚种10MR8的细菌细胞及无细胞滤液对念珠菌分离株的抗生物膜活性。
8MR7和副干酪亚种10MR8的细菌细胞对生物膜的抑制作用不太明显，而观察到这些细菌的无细胞滤液可抑制念珠菌菌株生物膜的形成。虽然抑制生物膜形成的主要机制是竞争黏附，但得出结论，乳酸菌无细胞滤液中所含物质也很重要。
这些分离株有望成为可用于医疗保健材料抗黏附目的的潜在细菌。
