Ogunshe Adenike A O, Omotoso Mopelola A, Bello Victoria B
Applied Microbiology and Infectious Diseases Unit, Department of Microbiology, Faculty of Science, University of Ibadan, Ibadan, Oyo State 900001, Nigeria.
Malays J Med Sci. 2011 Oct;18(4):13-25.
Research from developing countries, such as Nigeria, on Lactobacillus species in the female urogenital tract and their role as a barrier to vaginal infection is limited. Therefore, the aim of this study was to assess the clinical biotherapeutic potential of indigenous Lactobacillus species.
Antimicrobial metabolites production were characterised using simple and easily reproducible qualitative and quantitative methods. The in vitro inhibitory effect of Lactobacillus antimicrobials on vulvovaginal candidiasis-associated Candida species was investigated using modified agar spot and agar well-diffusion methods.
The maximum levels of lactic acid, hydrogen peroxide, and diacetyl from 20 vaginal Lactobacillus strains from diseased subjects were 1.46 mg/L, 1.36 mmol/L, and 1.72 mg/L respectively. From the 4 healthy subjects, the maximum level of lactic acid was 1.08 mg/L; hydrogen peroxide, 1.36 mmol/L; and diacetyl, 0.86 mg/L. The maximum productions of these substances occurred between 72 and 120 hours of incubation. The in vitro antagonistic activities of vaginal L. acidophilus, L. fermentum, L. brevis, L. plantarum, L. casei, L. delbrueckii, and L. jensenii from diseased subjects inhibited a maximum of 5.71% of the 35 Candida species tested, while vaginal L. acidophilus and L. plantarum from healthy subjects inhibited between 57.1% and 68.6% of Candida species in vitro.
Antimicrobial-producing lactobacilli can be considered as adjunct biotherapeutic candidates for the treatment of vulvovaginal candidiasis.
来自发展中国家（如尼日利亚）关于女性泌尿生殖道中乳酸杆菌属及其作为阴道感染屏障作用的研究有限。因此，本研究的目的是评估本地乳酸杆菌属的临床生物治疗潜力。
使用简单且易于重现的定性和定量方法对抗菌代谢产物的产生进行表征。采用改良的琼脂点样法和琼脂孔扩散法研究乳酸杆菌抗菌剂对外阴阴道念珠菌病相关念珠菌属的体外抑制作用。
来自患病受试者的20株阴道乳酸杆菌产生的乳酸、过氧化氢和双乙酰的最高水平分别为1.46毫克/升、1.36毫摩尔/升和1.72毫克/升。来自4名健康受试者的乳酸最高水平为1.08毫克/升；过氧化氢为1.36毫摩尔/升；双乙酰为0.86毫克/升。这些物质的最大产量出现在培养72至120小时之间。来自患病受试者的阴道嗜酸乳杆菌、发酵乳杆菌、短乳杆菌、植物乳杆菌、干酪乳杆菌、德氏乳杆菌和詹氏乳杆菌的体外拮抗活性最多抑制了所测试的35种念珠菌中的5.71%，而来自健康受试者的阴道嗜酸乳杆菌和植物乳杆菌在体外抑制了57.1%至68.6%的念珠菌。
产生抗菌物质的乳酸杆菌可被视为治疗外阴阴道念珠菌病的辅助生物治疗候选药物。
