Pei-Jie Zhang, Yan Cao, Ke Zhang, Yue-Lin Song, Jun L I, Li Tang
Key Laboratory of Ethnomedicine (Minzu University of China), Ministory of Education, School of Pharmacy, Minzu University of China Beijing 100081, China Modern Research Center for Traditional Chinese Medicine, School of Chinese Materia Medica,Beijing University of Chinese Medicine Beijing 100029, China.
Modern Research Center for Traditional Chinese Medicine, School of Chinese Materia Medica,Beijing University of Chinese Medicine Beijing 100029, China.
Zhongguo Zhong Yao Za Zhi. 2020 Oct;45(19):4658-4666. doi: 10.19540/j.cnki.cjcmm.20200416.202.
Artemisia rupestris is widely used as a folk medicine of Uygur and Kazak with blood and detoxification, deaccumulation stagnation, clearing heat digestion and other effects. Currently, the chemical profile of A. rupestris has not been disclosed, resulting in a great obstacle for the systematic clarification of the efficacy materials and the quality evaluations. In this paper, HPLC-IT-TOF-MS was deployed to characterize the chemical constituents in A. rupestris. As a result, a total of 124 compounds were detected in 75% ethanol extract of A. rupestris. By comparing with the reference compounds, seven chlorogenic acids, and four flavonoids as well as one sesquiterpenoid were definitely identified. Moreover, twenty sesquiterpenes, sixty-five flavonoids and twenty-three chlorogenic acids were preliminarily identified by matching MS/MS spectral information with literature data and applying those empirical mass spectrometric cracking rules. In current study, the chemical composition of A. rupestris was profiled in depth, and the findings are envisioned to provide a theoretical basis for the further studies of this well-known herbal medicine, such as efficacy material characterization and quality assessment.
岩蒿作为维吾尔族和哈萨克族的民间药物被广泛使用，具有凉血解毒、消食化积、清热利湿等功效。目前，岩蒿的化学特征尚未公开，这给功效物质的系统阐明和质量评价带来了很大障碍。本文采用高效液相色谱-离子阱-飞行时间质谱（HPLC-IT-TOF-MS）对岩蒿中的化学成分进行表征。结果，在岩蒿75%乙醇提取物中总共检测到124种化合物。通过与对照品比较，明确鉴定出7种绿原酸、4种黄酮类化合物以及1种倍半萜类化合物。此外，通过将MS/MS光谱信息与文献数据匹配并应用那些经验性的质谱裂解规则，初步鉴定出20种倍半萜、65种黄酮类化合物和23种绿原酸。在本研究中，对岩蒿的化学成分进行了深入剖析，这些发现有望为进一步研究这种著名草药提供理论依据，如功效物质表征和质量评估。
