Agricultural Research Council - Onderstepoort Veterinary Institute. Onderstepoort, Pretoria, South Africa.
Faculty of Veterinary Sciences, University of Pretoria, Onderstepoort, Pretoria, South Africa.
J Virol Methods. 2021 Jan;287:114003. doi: 10.1016/j.jviromet.2020.114003. Epub 2020 Oct 23.
The last major Rift Valley fever outbreak in South Africa was between 2008 and 2011. Viruses isolated between 2008 and 2010 were phylogenetically assigned to Lineage C, Lineage K and the novel lineage H. The 2011 outbreaks occurred primarily in the Eastern, Western and Northern Cape provinces, with no sequence data or phylogenetic relationship published. Samples from these outbreaks were submitted to the Faculty of Veterinary Sciences, University of Pretoria, for immunohistochemical confirmation of Rift Valley fever phlebovirus presence. These samples were formalin-fixed and paraffin-embedded (FFPE) and stored at the Pathology section for several years. This study describes a modified extraction method used to obtain RNA from the FFPE samples, as well as the primer combinations used to phylogenetically classify them as belonging to the novel lineage H.
上一次南非大规模裂谷热疫情发生在 2008 年至 2011 年期间。2008 年至 2010 年分离的病毒在系统发生上被分配到 C 谱系、K 谱系和新型 H 谱系。2011 年的疫情主要发生在东开普省、西开普省和北开普省，但没有公布序列数据或系统发育关系。来自这些疫情的样本被提交给比勒陀利亚大学兽医学院，用于免疫组织化学确认裂谷热病毒的存在。这些样本经过福尔马林固定和石蜡包埋（FFPE），并在病理学部门保存了数年。本研究描述了一种改良的从 FFPE 样本中提取 RNA 的方法，以及用于对其进行系统发育分类以归属于新型 H 谱系的引物组合。
