Analytical Chemistry Department, Faculty of Chemical Sciences. Complutense University of Madrid, 28040, Madrid, Spain.
I3A, University of Zaragoza, Campus Rio Ebro, María de Luna 3, 50018, Zaragoza, Spain.
Food Chem Toxicol. 2020 Dec;146:111849. doi: 10.1016/j.fct.2020.111849. Epub 2020 Nov 6.
The intake of toxic compounds through the diet as a result of migration processes from food packaging is of increasing concern. It has been shown that the surfactant commercially known as surfynol, which is commonly used in food-contact materials, is capable of migrating from multilayer containers into the food, reaching potentially harmful concentration levels. In the present study, the integration of an untargeted and a targeted metabolomics approach has been carried out using NTERA-2 germinal cells as in-vitro model, to make further progress in elucidating the molecular mechanisms associated with the toxicity of surfynol. This study has allowed the identification of different altered metabolites mainly related with energy-acquiring, cell development and cellular defense mechanisms. While glutamine, L-threonine, propanoate, octadecanoate and carbamate were found at higher concentrations in cells exposed tu surfynol, L-valine, oxalate, phosphate, phenylalanine and myoinositol were found inhibited. Additionally, concentrations of ATP, ADP and NAD were found significantly inhibited, supporting the idea that surfynol induces glycolysis inactivation. The results obtained strengthen the evidence of the toxicity associated to surfynol; therefore, reinforcing the need for a more comprehensive study on the viability of its use in food packaging.
由于食品包装迁移过程，通过饮食摄入有毒化合物的问题越来越受到关注。研究表明，常用于食品接触材料的商业表面活性剂 Surfynol 能够从多层容器迁移到食品中，达到潜在的有害浓度水平。在本研究中，使用 NTERA-2 生殖细胞作为体外模型，结合非靶向和靶向代谢组学方法，进一步阐明了 Surfynol 毒性相关的分子机制。这项研究确定了不同的代谢物变化，主要与能量获取、细胞发育和细胞防御机制有关。在暴露于 Surfynol 的细胞中，谷氨酰胺、L-苏氨酸、丙酸盐、十八烷酸盐和氨基甲酸酯的浓度升高，而 L-缬氨酸、草酸盐、磷酸盐、苯丙氨酸和肌醇的浓度降低。此外，还发现 ATP、ADP 和 NAD 的浓度显著抑制，这支持了 Surfynol 诱导糖酵解失活的观点。研究结果进一步证实了 Surfynol 相关毒性的证据，因此需要对其在食品包装中的使用可行性进行更全面的研究。
