Bacteriology, Immunology and Mycology Department, Faculty of Veterinary Medicine, Suez Canal University, Egypt.
Animal Health Research Institute, Dokki, Giza, Egypt.
Comp Immunol Microbiol Infect Dis. 2020 Dec;73:101553. doi: 10.1016/j.cimid.2020.101553. Epub 2020 Sep 17.
Bovine tuberculosis is a devastating illness in cattle and it has the ability to transmit causing severe troubles in human. Mycobacterium bovis (M. bovis) infection in human indeed becomes increasingly critical especially in developing countries. Early diagnosis is very important to control and limit its spreading. The aim of this study is to examine the genetic differentiation and possibilities of transmission between cattle and human. Lymph node and sputum samples were collected from cattle and patients showing tuberculin test positive; respectively for phenotypic identification and for molecular examination by detection of IS6110 and oxyR genes which are specific for MTC and M. bovis; respectively. The phenotypic identification of sputum samples showed 80 % positive by both stain and culture, while, lymph nodes revealed 66 % and 84 % positive by stain and culture method; respectively. Alignment of oxyR gene sequences of M. tuberculosis and M. bovis was used as a feature for differentiation between the 2 genes in these two genetically closely similar microorganisms showed 99 % identities between the 2 genes. Alignment and phylogenetic analysis of Mpb70 gene sequences from animal and human origin showed very high relatedness (99.32 %) to each other confirming that the zoonotic transmission is most probably occurred.
牛型结核是一种严重影响牛类的疾病，并且它有能力在人类之间传播，导致严重的问题。人感染牛分枝杆菌（M. bovis）的情况确实变得越来越严重，尤其是在发展中国家。早期诊断对于控制和限制其传播非常重要。本研究旨在研究牛和人之间的遗传分化和传播的可能性。从表现结核菌素试验阳性的牛和患者中收集了淋巴结和痰液样本；分别进行表型鉴定和分子检测，以检测 IS6110 和 oxyR 基因，这些基因分别是 MTC 和 M. bovis 的特异性基因。痰液样本的表型鉴定显示，两种染色和培养方法的阳性率均为 80%，而淋巴结的阳性率分别为染色法的 66%和培养法的 84%。结核分枝杆菌和牛分枝杆菌 oxyR 基因序列的比对被用作区分这两种在遗传上非常相似的微生物的特征，这两种基因的相似度为 99%。从动物和人类来源的 Mpb70 基因序列的比对和系统发育分析表明，它们之间非常相似（99.32%），这证实了人畜共患病的传播很可能发生。
