Faculty of Material Science and Chemical Engineering, Ningbo University, Ningbo, 315211, China.
Institute of Pharmaceutical Analysis, College of Pharmacy, Jinan University, Guangzhou, 510632, China.
Talanta. 2021 Jan 15;222:121456. doi: 10.1016/j.talanta.2020.121456. Epub 2020 Aug 2.
It was extremely urgent to develop some simultaneous and sensitive biosensors for detecting multiplex serum tumor markers (TMs) for early screening of cancers. Herein, a multiplex assay was developed based on the DNA-LaMnO (DNA-LMO) perovskite encoded probes and targets mediated competitive replacement strategy. Alpha fetoprotein (AFP), carcinoembryonic antigen (CEA) and prostate specific antigen (PSA) markers were employed as representative target TMs. Aptasensor is prepared by a series of DNA-LMO-M encode probes which were prepared by three hyperbranched DNA firstly immobilized on LMO encapsulating Pb, Cd or Cu ions. Then, three TMs aptamers were labeled on the stirring-rod and hybridized with the probes. After the developed encoded probes was incubated the TMs, the encoded probes corresponding to different TMs can be released into the supernatant through the competitive replacement. The inner metal ion can be simultaneously detected by square wave voltammetry corresponding to various TMs. Since the stirring rod can enrich many encoded probes containing a lot of metal ions, multiplex signal amplification can be realized. Due to the enrichment and easy separation of the stirring rod, the signal-to-noise ratio was also obviously improved and thus to results in good sensitivity and accuracy. Moreover, it took only 20 min to detect three targets which much faster than many same types of aptasensor. Under the optimal conditions, the low detection limit for CEA (3.6 × 10 ng/mL), AFP (3.4 × 10 ng/mL) and PSA (2.8 × 10 ng/mL) were obtained. Therefore, this method is likely to be used for early and sensitive screening of tumors.
开发一些同时且灵敏的用于检测多重血清肿瘤标志物（TMs）的生物传感器对于癌症的早期筛查极其紧迫。在此，开发了一种基于 DNA-LaMnO（DNA-LMO）钙钛矿编码探针和目标介导的竞争取代策略的多重分析。甲胎蛋白（AFP）、癌胚抗原（CEA）和前列腺特异性抗原（PSA）标志物被用作代表性的靶标 TMs。通过首先固定在 LMO 上的三个超支 DNA 制备的一系列 DNA-LMO-M 编码探针制备了适体传感器，LMO 封装了 Pb、Cd 或 Cu 离子。然后，三个 TM 适体标记在搅拌棒上并与探针杂交。在开发的编码探针孵育 TM 后，对应于不同 TM 的编码探针可以通过竞争取代释放到上清液中。通过方波伏安法可以同时检测到对应于各种 TM 的内金属离子。由于搅拌棒可以富集含有大量金属离子的许多编码探针，因此可以实现多重信号放大。由于搅拌棒的富集和易于分离，信噪比也明显提高，从而实现了良好的灵敏度和准确性。此外，检测三个靶标仅需 20 分钟，比许多相同类型的适体传感器快得多。在最佳条件下，获得了对 CEA（3.6×10ng/mL）、AFP（3.4×10ng/mL）和 PSA（2.8×10ng/mL）的低检测限。因此，该方法可能用于肿瘤的早期和灵敏筛查。
