Research Center for Materials Science and Opti-Electronic Technology, University of Chinese Academy of Sciences, No.19A Yuquan Road, Beijing, 100049, PR China; School of Electronic, Electrical and Communication Engineering, University of Chinese Academy of Sciences, PR China.
School of Physics, University of Chinese Academy of Sciences, PR China.
Talanta. 2021 Jan 15;222:121466. doi: 10.1016/j.talanta.2020.121466. Epub 2020 Aug 2.
Glycated hemoglobin (HbA1c) has been widely explored as an important marker for monitoring and diagnosing diabetes. Due to the advantages of high selectivity, easy preparation, and convenient preservation of aptamers, research on glycated hemoglobin detection utilizing aptasensors has received much attention in recent years. However, factors such as the pH and the salt concentration of the solution and the structure of the aptamer could influence the interactions between HbA1c and the aptamer. In this study, the factors were evaluated using surface plasmon resonance (SPR). The results show that the pH and the salt concentration can greatly affect the formation of a complex between the aptamer and HbA1c. In the stereostructure of the aptamer, loop L1 may be an important motif for recognizing glycated hemoglobin. In addition, the best condition for detecting HbA1c was at pH 6, with a high sensitivity and a low limit of detection(LOD) (1.06 × 10RUnM /2.55 nM). The results also demonstrated that the use of an SPR aptamer biosensor can be a sensitive technique to improve the accuracy and correctness of HbA1c measurement.
糖化血红蛋白（HbA1c）已被广泛探索作为监测和诊断糖尿病的重要标志物。由于适配体具有高选择性、易于制备和方便保存的优点，近年来利用适配体传感器检测糖化血红蛋白的研究受到了广泛关注。然而，溶液的 pH 值和盐浓度以及适配体的结构等因素会影响 HbA1c 与适配体之间的相互作用。在这项研究中，使用表面等离子体共振（SPR）评估了这些因素。结果表明，pH 值和盐浓度会极大地影响适配体与 HbA1c 形成复合物的能力。在适配体的立体结构中，环 L1 可能是识别糖化血红蛋白的重要基序。此外，检测 HbA1c 的最佳条件为 pH 值 6，具有高灵敏度和低检测限（1.06×10^-2 nM/2.55 nM）。研究结果还表明，使用 SPR 适配体生物传感器可以作为一种敏感的技术，以提高 HbA1c 测量的准确性和正确性。
