Batzri S, Harmon J W, Dubois A, Moskowitz D, Weichbrod R, Rich N M
Department of Surgery, Uniformed Services, University of the Health Sciences, Bethesda, Maryland 20814.
J Surg Res. 1987 Nov;43(5):398-406. doi: 10.1016/0022-4804(87)90097-7.
A new model for measuring gastric secretory parameters in awake guinea pigs is described. A chronic cannula was surgically implanted in the stomach of each guinea pig. The rates of gastric secretion and changes in intragastric volume were measured using a dye dilution technique. In contrast to previous techniques in small laboratory animals, there was no collection of gastric juice via drainage, no oral intubation for aspiration was involved, no special or sophisticated equipment was used, no anesthesia was employed, and there was no stress associated with acute surgery. This method offers a valuable advantage by combining the chronic gastric cannula with a dye dilution technique in that the same animal can be used several times and finally, several gastric secretory parameters can be measured simultaneously. The animals were used from 3 weeks to 10 months after surgery and as many as 15 studies were performed on the same guinea pig. Samples were collected at 10-min intervals and analyzed for acid and dye concentration from which the onset and kinetics of gastric secretion were followed. Basal gastric secretion (11.8 +/- 1.6 mueq/kg/min; all mean +/- 1 SEM) was increased within 20 min after subcutaneous infusion of histamine (30 micrograms/kg/hr) and peaked by 40-60 min at a mean acid output rate of 41 +/- 3 mueq/kg/min. Histamine also increased the intragastric volume from 6.3 to 13.4 ml as it increased fluid output from 1.6 +/- 0.1 ml/10 min to 3.4 +/- 0.2 ml/10 min. The increase in acid output caused by histamine was inhibited by the H2-antagonists cimetidine (3 mumole/kg) and ranitidine at 0.5 mumole/kg. Omeprazole (1.2 mumole/kg), an H-K-ATPase inhibitor, almost abolished acid output under both basal and histamine-stimulated conditions. Thus, the present method is simple and suitable to study the physiology and pharmacology of gastric secretion in the guinea pig with a particular emphasis on the action of histamine. Furthermore, because of the species involved, there is also a significant economical advantage and the guinea pig can also be used as a potential model for studying experimental ulcer.
本文描述了一种用于测量清醒豚鼠胃分泌参数的新模型。通过手术在每只豚鼠的胃中植入一根慢性插管。使用染料稀释技术测量胃分泌速率和胃内体积变化。与先前用于小型实验动物的技术相比，该方法无需通过引流收集胃液，无需进行口腔插管抽吸，不使用特殊或复杂的设备，不采用麻醉，且不存在与急性手术相关的应激反应。该方法通过将慢性胃插管与染料稀释技术相结合，具有显著优势，即同一动物可多次使用，最终可同时测量多个胃分泌参数。动物在手术后3周龄至10月龄期间使用，同一豚鼠可进行多达15次研究。每隔10分钟采集样本，分析酸和染料浓度，从而追踪胃分泌的起始和动力学。皮下注射组胺（30微克/千克/小时）后20分钟内，基础胃分泌（11.8±1.6微当量/千克/分钟；所有均值±1标准误）增加，40 - 60分钟时达到峰值，平均酸分泌速率为41±3微当量/千克/分钟。组胺还使胃内体积从6.3毫升增加到13.4毫升，同时使液体分泌量从1.6±0.1毫升/10分钟增加到3.4±0.2毫升/10分钟。组胺引起的酸分泌增加被H2拮抗剂西咪替丁（3微摩尔/千克）和雷尼替丁（0.5微摩尔/千克）抑制。奥美拉唑（1.2微摩尔/千克），一种H - K - ATP酶抑制剂，在基础和组胺刺激条件下几乎完全消除了酸分泌。因此，本方法简单，适用于研究豚鼠胃分泌的生理学和药理学，尤其侧重于组胺的作用。此外，由于所涉及的物种，还具有显著的经济优势，豚鼠也可作为研究实验性溃疡的潜在模型。
