Jam Neda, Hajimohammadi Reza, Gharbani Parvin, Mehrizad Ali
Department of Chemical Engineering, Ahar Branch, Islamic Azad University, Ahar, Iran.
Department of Chemistry, Ahar Branch, Islamic Azad University, Ahar, Iran.
Saudi J Biol Sci. 2022 Mar;29(3):1730-1736. doi: 10.1016/j.sjbs.2021.10.057. Epub 2021 Oct 25.
The antibacterial effects of combined extracts of Punica granatum L. and Areca nut (P.A) against resistant bacteria, a gram-positive bacterium, and three gram-negative bacteria, and in individual and biofilm forms was studied. Antibacterial activity was studied using disk diffusion method, microbroth dilution, and microtiter plate methods. Given the disc diffusion test (Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC)), the extracts had inhibitory effects on the individual forms of bacteria. However, the ethanolic extract had greater effectiveness than the methanolic extract. Generally, ethanol efficiently extracts flavonoids and their glycosides, catechol and tannins. This fact due to the ethanol polarity that is equal 0.654. The results indicated that the ability of extracts in inhibiting the formation of biofilms, destruction of biofilms, and prevention of metabolic activity of bacteria had a direct relationship with concentration and the highest inhibitory was seen on (98.98%), (94.98%), and (88.55%). Based on the results, the P.A. combined extract can be used as an alternative combination with the ability to inhibit antibiotic-resistant bacteria in single and biofilm forms.
研究了石榴（Punica granatum L.）和槟榔（P.A）的联合提取物对耐药菌（一种革兰氏阳性菌和三种革兰氏阴性菌）及其单个和生物膜形式的抗菌作用。采用纸片扩散法、微量肉汤稀释法和微量滴定板法研究抗菌活性。根据纸片扩散试验（最低抑菌浓度（MIC）和最低杀菌浓度（MBC）），提取物对细菌的单个形式具有抑制作用。然而，乙醇提取物比甲醇提取物具有更高的有效性。一般来说，乙醇能有效地提取黄酮类化合物及其糖苷、儿茶酚和单宁。这是由于乙醇的极性等于0.654。结果表明，提取物抑制生物膜形成、破坏生物膜和防止细菌代谢活性的能力与浓度有直接关系，对金黄色葡萄球菌（98.98%）、大肠杆菌（94.98%）和铜绿假单胞菌（88.55%）的抑制作用最强。基于这些结果，P.A.联合提取物可作为一种替代组合，具有抑制单个和生物膜形式的抗生素耐药菌的能力。
