Sundaresan Srivignesh, Philosoph-Hadas Sonia, Riov Joseph, Salim Shoshana, Meir Shimon
Department of Postharvest Science, Agricultural Research Organization (ARO), The Volcani Center, Rishon LeZion 7528809, Israel.
The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 7610001, Israel.
Life (Basel). 2020 Nov 6;10(11):273. doi: 10.3390/life10110273.
The abscission process occurs in a specific abscission zone (AZ) as a consequence of the middle lamella dissolution, cell wall degradation, and formation of a defense layer. The proteins and metabolites related to these processes are secreted by vesicle trafficking through the plasma membrane to the cell wall and middle lamella of the separating cells in the AZ. We investigated this process, since the regulation of vesicle trafficking in abscission systems is poorly understood. The data obtained describe, for the first time, the kinetics of the upregulated expression of genes encoding the components involved in vesicle trafficking, occurring specifically in the tomato () flower AZ (FAZ) during pedicel abscission induced by flower removal. The genes encoding vesicle trafficking components included soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs), SNARE regulators, and small GTPases. Our results clearly show how the processes of protein secretion by vesicle trafficking are regulated, programmed, and orchestrated at the level of gene expression in the FAZ. The data provide evidence for target proteins, which can be further used for affinity purification of plant vesicles in their natural state. Such analyses and dissection of the complex vesicle trafficking networks are essential for further elucidating the mechanism of organ abscission.
脱落过程发生在特定的脱落区（AZ），这是中层溶解、细胞壁降解以及防御层形成的结果。与这些过程相关的蛋白质和代谢物通过囊泡运输穿过质膜分泌到AZ中分离细胞的细胞壁和中层。我们对这一过程进行了研究，因为在脱落系统中囊泡运输的调控仍知之甚少。所获得的数据首次描述了在去花诱导的番茄（）花梗脱落过程中，编码参与囊泡运输的成分的基因上调表达的动力学，该过程特别发生在番茄花脱落区（FAZ）。编码囊泡运输成分的基因包括可溶性N - 乙基马来酰亚胺敏感因子附着蛋白受体（SNAREs）、SNARE调节因子和小GTP酶。我们的结果清楚地表明了在FAZ中，基因表达水平上囊泡运输介导的蛋白质分泌过程是如何被调控、编程和协调的。这些数据为目标蛋白提供了证据，可进一步用于天然状态下植物囊泡的亲和纯化。对复杂囊泡运输网络的此类分析和剖析对于进一步阐明器官脱落机制至关重要。
