College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi, 712100, China.
College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi, 712100, China; Research Center of Recycle Agricultural Engineering and Technology of Shaanxi Province, Northwest A&F University, Yangling, Shaanxi, 712100, China.
J Environ Manage. 2021 Feb 1;279:111560. doi: 10.1016/j.jenvman.2020.111560. Epub 2020 Nov 7.
This study assessed the effects of superphosphate (SPP) and phosphogypsum (PPG) on the bacterial and fungal community succession and molecular ecological networks during composting. Adding SPP and PPG had positive effects on the bacterial richness and diversity, negative effects on the fungal richness and diversity. The microbial diversity and richness were higher in PPG than SPP. Non-metric multidimensional scaling analysis clearly separated SPP and PPG from the control treatment with no additives. The dominant genera comprised Turicibacter, Bacillus, norank_o_SBR1031, Thermobifida, norank_f_Limnochordaceae, Truepera, Thermopolyspora, Mycothermus, Dipodascus, Thermomyces, and unclassified_p_Ascomycota. In all treatments, the major bacterial species differed clearly in the later thermophilic, cooling, and maturation composting stages, whereas the main fungal species varied significantly in the thermophilic stage. The changes in the dominant microorganisms in SPP and PPG may have inhibited or promoted the degradation of organic matter during various composting stages. Adding SPP and PPG led to more complex bacterial networks and less complex fungal networks, where SPP had more adverse effects on the fungal networks than PPG. SPP and PPG could potentially alter the co-occurrence patterns of the bacterial and fungal communities by changing the most influential species. SPP and PPG changed the composition and succession of the microbial community by influencing different physiochemical properties during various composting stages where the pH was the main explanatory factor. Overall, this study provides new insights into the effects of SPP and PPG on the microbial community and its interactions during composting.
本研究评估了过磷酸钙（SPP）和磷石膏（PPG）对堆肥过程中细菌和真菌群落演替及分子生态网络的影响。添加 SPP 和 PPG 对细菌丰富度和多样性有积极影响，对真菌丰富度和多样性有消极影响。PPG 中的微生物多样性和丰富度高于 SPP。非度量多维尺度分析清楚地将 SPP 和 PPG 与无添加剂的对照处理区分开来。优势属包括 Turicibacter、Bacillus、norank_o_SBR1031、Thermobifida、norank_f_Limnochordaceae、Truepera、Thermopolyspora、Mycothermus、Dipodascus、Thermomyces 和未分类的 p_Ascomycota。在所有处理中，主要细菌种类在后期高温、冷却和成熟堆肥阶段明显不同，而主要真菌种类在高温阶段明显不同。SPP 和 PPG 中优势微生物的变化可能在不同堆肥阶段抑制或促进了有机物的降解。添加 SPP 和 PPG 导致细菌网络更加复杂，真菌网络更加简单，其中 SPP 对真菌网络的影响大于 PPG。SPP 和 PPG 通过改变最具影响力的物种，可能会改变细菌和真菌群落的共现模式。SPP 和 PPG 通过在不同堆肥阶段影响不同的理化性质来改变微生物群落的组成和演替，其中 pH 是主要的解释因素。总的来说，本研究为 SPP 和 PPG 对堆肥过程中微生物群落及其相互作用的影响提供了新的见解。
