胃癌中脂联素的表达及血清水平
Expression of and and Serum Level of Adiponectin in Gastric Cancer.
作者信息
Kordafshari Morteza, Nourian Mahyar, Mehrvar Narjes, Jalaeikhoo Hassan, Etemadi Aida, Khoshdel Ali Reza, Idris Mohammad Ghaznavi, Iravani Shahrokh, Mehrvar Azim
机构信息
Research Center for Cancer Screening and Epidemiology, AJA University of Medical Sciences, Tehran, Iran.
Mahak Hematology Oncology Research Center (Mahak-HORC), Mahak Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
出版信息
Gastrointest Tumors. 2020 Oct;7(4):103-109. doi: 10.1159/000510342. Epub 2020 Sep 16.
BACKGROUND
Cancer is one of the major causes of death worldwide and the third leading cause of death in Iran. One of the proteins that are considered having anticancer effects is the adiponectin hormone. Adiponectin leads to programmed cell death, prevents cell growth and proliferation, and increases the expression levels of .
AIM
The aim of this study was to assay the expression of adiponectin receptors ( and ) genes in gastric cancer patients.
MATERIALS AND METHODS
In this case-control study, 42 gastric cancer patients and 52 volunteers as healthy controls were enrolled. Total RNA was extracted. cDNA was synthesized by the reverse transcription method, and expression analysis was performed by real-time PCR. The serum level of adiponectin was also measured by ELISA.
RESULTS
The expression of both and was significantly higher than the control group ( = 0.02). Serum adiponectin was significantly lower in gastric cancer cases when compared with normal controls ( = 0.03).
CONCLUSION
We found that expression level of and is strongly higher; however, the level of circulating adiponectin is lower in gastric cancer. Our study suggests that the expression of and , besides the low level of adiponectin, may play an important role in the development and/or progression of gastric cancer.
背景
癌症是全球主要死因之一，也是伊朗第三大死因。脂联素激素是被认为具有抗癌作用的蛋白质之一。脂联素可导致程序性细胞死亡，阻止细胞生长和增殖，并增加……的表达水平。
目的
本研究旨在检测胃癌患者脂联素受体（和）基因的表达。
材料与方法
在这项病例对照研究中，纳入了42例胃癌患者和52名作为健康对照的志愿者。提取总RNA。通过逆转录方法合成cDNA，并通过实时PCR进行表达分析。还通过ELISA测定血清脂联素水平。
结果
和的表达均显著高于对照组（=0.02）。与正常对照组相比，胃癌病例的血清脂联素显著降低（=0.03）。
结论
我们发现和的表达水平明显更高；然而，胃癌患者循环脂联素水平较低。我们的研究表明，和的表达以及脂联素水平较低可能在胃癌的发生和/或发展中起重要作用。
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