Hua Bin, Li Zhouwen, Gao Weikai, Feng Huilin, Chen Nan, Li Jiayi, Ji Xiaoming, Zhang Lu, Wu Zhiyong, Yan Shen, Ren Tianbao, Xu Chensheng, Liu Guoshun
Tobacco college, Henan Agricultural University, Zhengzhou, 450002, China.
Biochar Technology Engineering Laboratory of Henan Province, Zhengzhou, 450002, China.
Biotechnol Lett. 2021 Mar;43(3):655-666. doi: 10.1007/s10529-020-03046-1. Epub 2020 Nov 11.
Excessive application of chemical fertilizer and continuous cropping in plastic greenhouse resulted in soil quality decline. The decrease of soil C/N ratio and the imbalance of soil carbon pool structure have brought new challenges to soil health, crop yield and sustainable agricultural development.
The experiment was set up to explore the effect of modified biochar on soil bacterial community structure, and the correlation between soil environmental factors and bacterial community structure changes. Based on the plot experiment in the field, the effect of modified biochar was studied via high-throughput MiSeq sequencing.
Compared with the control (CK), the modified biochar (T) significantly increased soil water content, microbial biomass carbon (MBC), microbial biomass nitrogen (MBN) content and the ratio of MBC and MBN by 7.92%, 24.58%, 2.07% and 18.95%. Diversity index analysis showed that the application of modified biochar significantly increased the Shannon index, ACE index and Chao1 index of the bacterial community by 3.05%, 5.07% and 5.24%. Compared with the control, the modified biochar decreased the relative abundance of Actinobacteriota and Chloroflex by 6.81% and 2.19%, and increased the relative abundance of Proteobacteria and Acidobacteriota by 7.34% and 12.52%. Correlation analysis shows that soil bulk density and water content may be important related factors that affect bacterial community structure.
This study provides a theoretical basis for the directional control of modified biochar in the soil microecological environment in plastic greenhouse, which is conducive to healthy and sustainable farming.
塑料大棚中化肥过度施用和连作导致土壤质量下降。土壤碳氮比降低和土壤碳库结构失衡给土壤健康、作物产量和农业可持续发展带来了新挑战。
本试验旨在探究改性生物炭对土壤细菌群落结构的影响，以及土壤环境因子与细菌群落结构变化之间的相关性。基于田间小区试验，通过高通量MiSeq测序研究改性生物炭的效果。
与对照（CK）相比，改性生物炭（T）显著提高了土壤含水量、微生物量碳（MBC）、微生物量氮（MBN）含量以及MBC与MBN的比值，分别提高了7.92%、24.58%、2.07%和18.95%。多样性指数分析表明，施用改性生物炭显著提高了细菌群落的香农指数、ACE指数和Chao1指数，分别提高了3.05%、5.07%和5.24%。与对照相比，改性生物炭使放线菌门和绿弯菌门的相对丰度分别降低了6.81%和2.19%，使变形菌门和酸杆菌门的相对丰度分别提高了7.34%和12.52%。相关性分析表明，土壤容重和含水量可能是影响细菌群落结构的重要相关因素。
本研究为塑料大棚土壤微生态环境中改性生物炭的定向调控提供了理论依据，有利于健康和可持续耕作。
