Department of Theriogenology, Veterinary Medicine Faculty, Mansoura University, Mansoura, Egypt.
Clinical Pathology, Veterinary Medicine Faculty, Mansoura University, Mansoura, Egypt.
Reprod Domest Anim. 2021 Jan;56(1):142-152. doi: 10.1111/rda.13858. Epub 2020 Nov 22.
The aims of the present study were to determine uterine, vaginal and placental blood flows by Doppler ultrasound cross-buffalo gestation and to evaluate the relationships among reproductive Doppler parameters and serum metabolic parameters as well as oxidative stress. Uterine (UA) and vaginal (VA) arteries were scanned every month, and placentome was scanned from month 4 till 8 in gestation. Time-averaged maximum velocity (TAMV), pulsatility index (PI), resistance index (RI), systolic/diastolic ratio (SD) and arterial diameter (AD) were used for accessing UA and VA hemodynamics. Time-averaged maximum velocity positively correlated with and AD, and both negatively correlated with their PI, RI and SD in UA and VA. TAMV and AD increased constantly in pregnancy, with maximum increase in months 4 and 9. Pulsatility index, RI and AD of UA decreased between months 4 and 9, while PI, RI and AD of VA decreased between months 5 and 9 and then increased in month 10 in pregnancy. Time-averaged maximum velocity of placentome blood flow increased exponentially from months 4 to 8, but decreased at the last two months in pregnancy. Serum lipids were significantly higher in the first month compared to all other months, while glucose was significantly lower in months 9 and 10. Malondialdehyde increased from month 3 till term, but peaked in month 5 and 10. Glutathione and catalase were highest in the first month and remained after. Time-averaged maximum velocity and AD for both UA and VA negatively correlated with serum lipids, glucose, catalase and glutathione, while positively correlated with malondialdehyde and total protein. Thus, increases in uterine blood flow (UtBF), vaginal blood flow (VaBF) and placental blood flow (PaBF) are associated with increased metabolism and oxidative stress in buffalo pregnancy.
本研究旨在通过多普勒超声交叉水牛妊娠来确定子宫、阴道和胎盘血流，并评估生殖多普勒参数与血清代谢参数以及氧化应激之间的关系。每月扫描子宫（UA）和阴道（VA）动脉，并从妊娠第 4 个月到第 8 个月扫描胎盘。用于评估 UA 和 VA 血液动力学的时间平均最大速度（TAMV）、搏动指数（PI）、阻力指数（RI）、收缩/舒张比（SD）和动脉直径（AD）。UA 和 VA 中的 TAMV 与 AD 呈正相关，与 PI、RI 和 SD 呈负相关。TAMV 和 AD 在妊娠期间持续增加，最大增加发生在第 4 和第 9 个月。UA 中的 PI、RI 和 AD 在第 4 至第 9 个月之间降低，而 VA 中的 PI、RI 和 AD 在第 5 至第 9 个月之间降低，然后在妊娠第 10 个月增加。胎盘血流的 TAMV 从第 4 个月到第 8 个月呈指数增加，但在妊娠的最后两个月减少。与所有其他月份相比，血清脂质在第一个月显著升高，而葡萄糖在第 9 和第 10 个月显著降低。丙二醛从第 3 个月到足月增加，但在第 5 和第 10 个月达到峰值。谷胱甘肽和过氧化氢酶在第一个月最高，之后保持不变。UA 和 VA 的 TAMV 和 AD 与血清脂质、葡萄糖、过氧化氢酶和谷胱甘肽呈负相关，与丙二醛和总蛋白呈正相关。因此，水牛妊娠中子宫血流（UtBF）、阴道血流（VaBF）和胎盘血流（PaBF）的增加与代谢和氧化应激的增加有关。
