Bone Research Lab, Department of Basic and Oral Biology, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil.
Department of Oral and Maxillofacial Surgery and Periodontology, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil.
Int J Exp Pathol. 2020 Dec;101(6):277-288. doi: 10.1111/iep.12379. Epub 2020 Nov 11.
Periodontal disease and osteoporosis are characterized by bone resorption, and researchers have shown an association between these two diseases through increasing loss of systemic bone mass and triggering alveolar bone loss. Green tea is a common and easily accessible beverage, and evidences show that flavonoid epigallocatechin gallate (EGCG) could decrease bone loss in pathologies such as osteoporosis and periodontal disease. In order to verify its possible effects and apply them in the treatment and prevention of these diseases, this investigation aimed to evaluate the influence of green tea extract (GTE) on bone metabolism of ovariectomized rats after experimental periodontal disease (EPD) by histological, morphological and microtomographic parameters. Wistar female rats were divided into Sham, Sham + EPD, Sham + EPD + GTE, OVX, OVX + EPD and OVX + EPD + GTE groups. Immediately after surgery, gavage administration of 50 mg/kg of green tea extract (GTE) was performed for 60 days, with subsequent induction of periodontal disease by ligature 15 days before euthanasia. Mandible and femur samples were collected for histological, morphometric and microtomographic analysis. The results were analysed by means of statistical software with significance set at 5%. Histological and morphometric analysis showed a significant decrease in alveolar and femoral trabecular bone loss in groups that received GTE. Microtomographic results showed that trabecular thickness and bone surface density values in alveolar bone interradicular septum of the OVX + EPD + GTE groups were similar to the Sham group. The results obtained suggest that green tea extract may improve bone metabolism in osteoporotic rats with periodontal disease.
牙周病和骨质疏松症的特征均为骨吸收，研究人员通过观察到系统性骨量丢失增加和触发牙槽骨丢失，发现这两种疾病之间存在关联。绿茶是一种常见且易于获得的饮料，有证据表明，黄烷醇表没食子儿茶素没食子酸酯（EGCG）可减少骨质疏松症和牙周病等疾病中的骨丢失。为了验证其可能的作用，并将其应用于这些疾病的治疗和预防，本研究旨在通过组织学、形态学和显微 CT 参数评估绿茶提取物（GTE）对实验性牙周病（EPD）后去卵巢大鼠骨代谢的影响。Wistar 雌性大鼠分为假手术组（Sham）、假手术+牙周病组（Sham+EPD）、假手术+牙周病+绿茶提取物组（Sham+EPD+GTE）、去卵巢组（OVX）、去卵巢+牙周病组（OVX+EPD）和去卵巢+牙周病+绿茶提取物组（OVX+EPD+GTE）。手术后立即进行 50mg/kg 的绿茶提取物（GTE）灌胃，60 天后用结扎法诱导牙周病，在安乐死前 15 天处死大鼠。收集下颌骨和股骨样本进行组织学、形态计量学和显微 CT 分析。结果采用统计软件进行分析，设定显著性水平为 5%。组织学和形态计量学分析显示，GTE 治疗组牙槽骨和股骨小梁骨丢失明显减少。显微 CT 结果显示，OVX+EPD+GTE 组牙槽骨骨小梁间的骨小梁厚度和骨表面密度值与 Sham 组相似。结果表明，绿茶提取物可能改善患有牙周病的骨质疏松症大鼠的骨代谢。
