Industrial Bio-materials Research Center, KRIBB, 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.
Department of Biotechnology, KRIBB School of Bioscience, Korea University of Science and Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon 34113, Republic of Korea.
Int J Syst Evol Microbiol. 2020 Dec;70(12):6476-6481. doi: 10.1099/ijsem.0.004560.
A Gram-stain-positive, facultative anaerobic, rod-shaped bacteria isolated from the small intestine of a mini pig was designated as strain YH-lac9. 16S rRNA gene sequence analysis revealed that the strain belongs to the genus and is closely related to JCM 17472, JCM 15042 and JCM 13927, with 97.6, 96.2 and 95.7 % sequence similarity, respectively. Analysis of housekeeping gene sequences ( and ) revealed that the strain formed a sub-cluster with , supporting the results of 16S rRNA gene sequences analysis. The average nucleotide identity value for YH-lac9 and the most closely related strain is 74.1 %. The main fatty acids are Cω9, summed feature 7, C and summed feature 8. The G+C content of the genomic DNA is 37.8 mol%. In view of its chemotaxonomic, phenotypic and phylogenetic properties, YH-lac9 (=KCTC 25005=JCM 33997) represents a novel taxon. The name sp. nov. is proposed.
从迷你猪的小肠中分离到的一株革兰氏阳性、兼性厌氧、杆状细菌被命名为 YH-lac9 菌株。16S rRNA 基因序列分析表明，该菌株属于 属，与 JCM 17472、 JCM 15042 和 JCM 13927 密切相关，序列相似性分别为 97.6%、96.2%和 95.7%。系统发育分析基于管家基因（ 和 ）序列的结果与 16S rRNA 基因序列分析的结果一致，表明该菌株与 形成了一个亚群。YH-lac9 与最密切相关菌株的平均核苷酸同一性值为 74.1%。主要脂肪酸为 Cω9、summed feature 7、C16:0 和 summed feature 8。基因组 DNA 的 G+C 含量为 37.8mol%。鉴于其化学分类学、表型和系统发育特性，YH-lac9（=KCTC 25005=JCM 33997）代表了一个新的分类单元。提议使用 sp. nov. 作为该新种的名称。
