ABS Research Support Center, KRIBB, 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.
Vitabio, Inc., Daejeon, Republic of Korea.
Int J Syst Evol Microbiol. 2024 Feb;74(2). doi: 10.1099/ijsem.0.006284.
Two cocci-shaped, facultatively anaerobic, Gram-positive bacteria isolated from the faeces of a pig were designated as strains YH-aer221 and YH-aer222. Analysis of the 16S rRNA gene sequences revealed that the isolates were most closely related to JCM 18035 with 96.6 % similarity. The multi-locus sequence tree revealed that the isolates formed a sub-cluster adjacent to JCM 18035. The average nucleotide identity values for the isolates and their most closely related strains were 71.8 and 71.7 %, respectively; and the digital DNA-DNA hybridization values for the isolates and their most closely related strains were 25.6 and 25.5 %, respectively. The main fatty acids were Cω9, C and C. The cell wall contained the -diaminopimelic acid-based peptidoglycan. The two isolates shared the same metabolic pathways. Isolates YH-aer221 and YH-aer222 harboured the same CRISPR array with 33 and 46 spacers, respectively. Single-genome vs. metagenome analysis showed that the genomes of the isolates were not found in the available metagenome database. Given their chemotaxonomic, phenotypic and phylogenetic properties, YH-aer221 (= KCTC 25571=JCM 35699) and YH-aer222 (=KCTC 25573=JCM 35700) represent a novel taxon. The name sp. nov. is proposed.
从猪粪便中分离到两株兼性厌氧、球形、革兰氏阳性细菌，分别命名为 YH-aer221 和 YH-aer222 菌株。16S rRNA 基因序列分析表明，这两株菌与 JCM 18035 最为密切，相似性为 96.6%。多位序列树分析表明，这两株菌与 JCM 18035 形成了一个亚群。这两株菌与其最密切相关菌株的平均核苷酸同一性值分别为 71.8%和 71.7%；数字 DNA-DNA 杂交值分别为 25.6%和 25.5%。主要脂肪酸为 Cω9、C 和 C。细胞壁含有基于二氨基庚二酸的肽聚糖。这两株菌具有相同的代谢途径。两株菌都含有相同的 CRISPR 数组，分别有 33 和 46 个间隔区。单基因组与宏基因组分析表明，这两株菌的基因组在现有的宏基因组数据库中没有发现。鉴于它们的化学分类学、表型和系统发育特性，YH-aer221（=KCTC 25571=JCM 35699）和 YH-aer222（=KCTC 25573=JCM 35700）代表了一个新的分类单元。建议使用 sp. nov. 来命名。
