Paek Jayoung, Bai Lu, Shin Yeseul, Kim Hongik, Kook Joong-Ki, Kim Si Hyun, Shin Jeong Hwan, Chang Young Hyo
ABS Research Support Center, KRIBB, 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.
Vitabio Inc., Daejeon, 305-500, Republic of Korea.
Int J Syst Evol Microbiol. 2022 Nov;72(11). doi: 10.1099/ijsem.0.005617.
A lactic acid bacteria isolated from pig faeces was characterized using a polyphasic approach. Cells of the strain were Gram-stain-positive, rod-shaped and facultative anaerobic. Phylogenetic analysis of 16S rRNA gene sequence indicated that the isolate belonged to the genus ; however, the similarity to other homologues within the genus was <98 %. Analysis of housekeeping gene sequences ( and ) revealed that the strain formed a sub-cluster adjacent to and . The main fatty acids of the strain is the Cω9 and C. The G+C content of the genomic DNA was 62.8 mol %. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, aminophospholipids and phospholipids. The cell-wall peptidoglycan did not contain -diaminopimelic acid. Thus, YH-lac21 (=KCTC 21185=JCM 34953) represents a novel species. The name sp. nov. is proposed.
采用多相分类法对从猪粪便中分离出的一株乳酸菌进行了鉴定。该菌株细胞革兰氏染色阳性，呈杆状，兼性厌氧。16S rRNA基因序列的系统发育分析表明，该分离株属于 属；然而，与该属内其他同源物的相似性<98%。看家基因序列（ 和 ）分析表明，该菌株形成了一个与 和 相邻的亚簇。该菌株的主要脂肪酸为Cω9和C。基因组DNA的G+C含量为62.8 mol%。主要极性脂为双磷脂酰甘油、磷脂酰甘油、氨基磷脂和磷脂。细胞壁肽聚糖不含 -二氨基庚二酸。因此，YH-lac21（=KCTC 21185=JCM 34953）代表一个新物种。提议将其命名为 新种。
