酵母中葡萄糖代谢的比较放射性呼吸测定研究。

A comparative radiorespirometric study of glucose metabolism in yeasts.

作者信息

Bruinenberg P M, Waslander G W, van Dijken J P, Scheffers W A

机构信息

Department of Microbiology and Enzymology, Delft University of Technology, The Netherlands.

出版信息

Yeast. 1986 Jun;2(2):117-21. doi: 10.1002/yea.320020207.

DOI:10.1002/yea.320020207

PMID:3333300

Abstract

A comparative radiorespirometric study of glucose metabolism in glucose-limited chemostat cultures of Saccharomyces cerevisiae, Candida utilis and Rhodosporidium toruloides was performed in an attempt to estimate the contribution of the hexose monophosphate (HMP) pathway to glucose metabolism. Radioactively labelled glucose was administered directly to the cultures in a constant substrate feed, without disturbance of the steady state. The 14CO2 yields from [1-14C]- and [6-14C]-glucose demonstrated that the HMP pathway activities for the three yeasts were very similar. Furthermore, a quantitative analysis of results indicated that the HMP pathway activities were close to the theoretical minimum needed to cover the NADPH requirement for biomass formation.

摘要

对酿酒酵母、产朊假丝酵母和红酵母在葡萄糖受限恒化器培养中的葡萄糖代谢进行了比较放射性呼吸测定研究，以试图估算己糖磷酸（HMP）途径对葡萄糖代谢的贡献。将放射性标记的葡萄糖在恒定底物进料中直接加入培养物，而不干扰稳态。[1-¹⁴C]-和[6-¹⁴C]-葡萄糖产生的¹⁴CO₂表明，这三种酵母的HMP途径活性非常相似。此外，结果的定量分析表明，HMP途径活性接近满足生物量形成所需NADPH的理论最小值。