Technology Division, DOE Joint BioEnergy Institute, Emeryville, California, United States of America.
Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California, United States of America.
PLoS One. 2020 Nov 11;15(11):e0242157. doi: 10.1371/journal.pone.0242157. eCollection 2020.
Microfluidic applications have expanded greatly over the past decade. For the most part, however, each microfluidics platform is developed with a specific task in mind, rather than as a general-purpose device with a wide-range of functionality. Here, we show how a microfluidic system, originally developed to investigate protein phase behavior, can be modified and repurposed for another application, namely DNA construction. We added new programable controllers to direct the flow of reagents across the chip. We designed the assembly of a combinatorial Golden Gate DNA library using TeselaGen DESIGN software and used the repurposed microfluidics platform to assemble the designed library from off-chip prepared DNA assembly pieces. Further experiments verified the sequences and function of the on-chip assembled DNA constructs.
微流控应用在过去十年中得到了极大的扩展。然而，在大多数情况下，每个微流控平台都是针对特定任务开发的，而不是作为具有广泛功能的通用设备。在这里，我们展示了一个微流控系统如何进行修改和重新用于另一个应用，即 DNA 构建。我们添加了新的可编程控制器来引导试剂在芯片上流动。我们使用 TeselaGen DESIGN 软件设计了组合 Golden Gate DNA 文库的组装，并使用重新利用的微流控平台从外部制备的 DNA 组装片段组装设计的文库。进一步的实验验证了芯片上组装的 DNA 构建物的序列和功能。
