Laboratoire de Parasitologie, Univ. Manouba, Institution de la Recherche et de l'Enseignement Supérieur Agricoles, Ecole Nationale de Médecine Vétérinaire de Sidi Thabet, 2020, Sidi Thabet, Tunisia.
Friedrich-Loeffler-Institute, Institute of Novel and Emerging Infectious Diseases, Südufer 10, 17493 Greifswald, Insel Riems, Germany.
Ticks Tick Borne Dis. 2021 Jan;12(1):101601. doi: 10.1016/j.ttbdis.2020.101601. Epub 2020 Oct 25.
Crimean-Congo haemorrhagic fever is a viral tick-borne zoonotic disease caused by a Nairovirus, Crimean-Congo haemorrhagic fever virus (CCHFV). The present survey aimed to determine the exposure of one-humped camels (Camelus dromedarius) from southern Tunisia to CCHFV. A total of 273 sera from extensively reared camels were collected from Tataouine district, Tunisia, and tested by CCHFV-specific enzyme linked immunosorbent assays. By combining the results of three serological tests, the overall seroprevalence of CCHFV was estimated as 89.7% (245/273). No viral RNA was detected from camel sera using quantitative real-time PCR (RT-qPCR). A total of 165 ticks were collected from camels and tested with RT-qPCR, and only one Hyalomma impeltatum tick was positive for virus RNA.
克里米亚-刚果出血热是一种由纳罗病毒引起的病毒性蜱传动物源性疾病，即克里米亚-刚果出血热病毒（CCHFV）。本调查旨在确定突尼斯南部单峰驼（Camelus dromedarius）对 CCHFV 的暴露情况。从突尼斯的 Tataouine 地区采集了总共 273 份广泛饲养的骆驼血清，并通过 CCHFV 特异性酶联免疫吸附测定进行了检测。通过结合三种血清学检测的结果，总体估计 CCHFV 的血清阳性率为 89.7%（245/273）。使用实时定量 RT-PCR（RT-qPCR）从骆驼血清中未检测到病毒 RNA。从骆驼身上共采集了 165 只蜱，并通过 RT-qPCR 进行了检测，只有一只伊氏革蜱呈病毒 RNA 阳性。
