Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary Medicine, Academy of Military Medical Sciences, Academy of Military Sciences, Changchun, China.
Key Laboratory of Ministry of Education for Protection and Utilization of Special Biological Resources in the Western China, Ningxia University, Yinchuan, China.
Vector Borne Zoonotic Dis. 2020 Jun;20(6):461-467. doi: 10.1089/vbz.2019.2529. Epub 2020 Mar 9.
Crimean-Congo hemorrhagic fever (CCHF) is a highly lethal infectious disease in humans caused by tick-borne Crimean-Congo hemorrhagic fever virus (CCHFV). To determine the potential risk of CCHF in the pastoral area of Northwestern China, the presence of antibody to CCHFV in the sera of two most common tick hosts, sheep and camels, in Inner Mongolia of China was evaluated. The purified recombinant nucleocapsid protein (NP) of CCHFV was prepared from a prokaryotic vector expressing the NP encoding gene, which was employed as the antigen to probe antibody against CCHFV in tick vectors using an immunoblotting assay. In total, 58.3% (35/60) and 54% (12/22) of camels were positive of antibody to CCHFV in sera at Alxa Youqi County and Ulan Hudu Gacha Village of Inner Mongolia Province, respectively. However, only 6.7% (2/30) of sera of sheep were determined positive in antibody to CCHFV in the Wulan Hudug check area in this study. Consequently, these results indicate that 54-58.3% camels were infected by CCHFV after exposure to tick bites in Inner Mongolia, which was significantly higher than 6.7% of infection in the sheep in this area, suggesting there is a certain relationship between the serological reactivity and exposure time to ticks, range of activity, living behaviors, and breeding time. Further intensive surveillance of livestock and exposed population is required to better understand the spread of CCHFV in this area.
克里米亚-刚果出血热（CCHF）是一种由蜱传播的克里米亚-刚果出血热病毒（CCHFV）引起的人类高度致命传染病。为了确定中国西北地区牧区发生克里米亚-刚果出血热的潜在风险，评估了中国内蒙古两种最常见的蜱宿主（绵羊和骆驼）血清中 CCHFV 抗体的存在情况。从表达 NP 编码基因的原核载体中制备了纯化的重组核衣壳蛋白（NP），该蛋白被用作抗原，通过免疫印迹分析探测蜱载体中针对 CCHFV 的抗体。在内蒙古阿拉善右旗和乌兰呼都格村，分别有 58.3%（35/60）和 54%（12/22）的骆驼血清抗体呈阳性。然而，在本研究的乌兰呼都格检查区，仅 6.7%（2/30）的绵羊血清抗体呈阳性。因此，这些结果表明，在内蒙古，54-58.3%的骆驼在被蜱叮咬后感染了 CCHFV，这明显高于该地区绵羊 6.7%的感染率，表明血清反应性与暴露于蜱的时间、活动范围、生活行为和繁殖时间之间存在一定关系。需要对牲畜和暴露人群进行更密集的监测，以更好地了解该地区 CCHFV 的传播情况。
