Guimarães Victor Hugo Dantas, Lelis Deborah de Farias, Oliveira Luis Paulo, Borém Luciana Mendes Araújo, Guimarães Felipe Alberto Dantas, Farias Lucyana Conceição, de Paula Alfredo Mauricio Batista, Guimarães André Luiz Sena, Santos Sérgio Henrique Sousa
Laboratory of Health Science, Postgraduate Program in Health Science, Universidade Estadual de Montes Claros (Unimontes), Montes Claros, Brasil.
Department of Medicine, Faculdades Integradas Pitágoras, Montes Claros, Brasil.
Arch Physiol Biochem. 2023 Apr;129(2):449-459. doi: 10.1080/13813455.2020.1835986. Epub 2020 Nov 11.
Diet macronutrient heterogeneity hinders animal studies' data extrapolation from metabolic disorders to human diseases.
The present study aimed to evaluate different fat-diet compositions' effect on inducing lipid/glucose metabolism alterations in mice.
Swiss male mice were fed for 12 weeks with five different diets: Standard Diet (ST), American Institute of Nutrition 93 for growth (AIN93G) high-butter/high-sugar (HBHS), high-lard/high-sugar (HLHS), and high-oil/high-sugar diet (soybean oil) (HOHS). Several parameters, such as serum biochemistry, histology, and liver mRNA expression, were accessed.
The main findings revealed that the HLHS diet dramatically altered liver metabolism inducing hepatic steatosis and increased total cholesterol, triglycerides, VLDL, increasing liver CCAAT/enhancer binding protein (CEBP-α), Acetyl-CoA carboxylase (ACC) and Catalase (CAT) mRNA expression. Moreover, the HLHS diet increased glucose intolerance and reduced insulin sensitivity.
High-fat/high-sugar diets are efficient to induce obesity and metabolic syndrome-associated alterations, and diets enriched with lard and sugar showed more effective results.
饮食中宏量营养素的异质性阻碍了动物研究数据从代谢紊乱向人类疾病的外推。
本研究旨在评估不同脂肪饮食组成对诱导小鼠脂质/葡萄糖代谢改变的影响。
将瑞士雄性小鼠用五种不同的饮食喂养12周：标准饮食（ST）、美国营养学会93号生长用饮食（AIN93G）、高黄油/高糖（HBHS）、高猪油/高糖（HLHS）和高油/高糖饮食（大豆油）（HOHS）。检测了几个参数，如血清生化、组织学和肝脏mRNA表达。
主要研究结果显示，HLHS饮食显著改变肝脏代谢，导致肝脂肪变性，并增加总胆固醇、甘油三酯、极低密度脂蛋白，增加肝脏CCAAT/增强子结合蛋白（CEBP-α）、乙酰辅酶A羧化酶（ACC）和过氧化氢酶（CAT）的mRNA表达。此外，HLHS饮食增加了葡萄糖不耐受性并降低了胰岛素敏感性。
高脂肪/高糖饮食能有效诱导肥胖和代谢综合征相关改变，富含猪油和糖的饮食显示出更有效的结果。
