Department for Public Health Microbiology, National Laboratory for Health, Environment and Food, Ljubljana, Slovenia.
Department for Public Health Microbiology, National Laboratory for Health, Environment and Food, Ljubljana, Slovenia.
Sci Total Environ. 2021 Feb 10;755(Pt 2):143226. doi: 10.1016/j.scitotenv.2020.143226. Epub 2020 Oct 28.
Previous studies on SARS-CoV and MERS-CoV reported the detection of viral RNA in the stool of both symptomatic and asymptomatic individuals. These clinical observations suggest that municipal and hospital wastewater from affected communities may contain SARS-CoV-2 RNA. Recent studies have also reported the presence of SARS-CoV-2 RNA in human feces. Wastewater-based epidemiology (WBE) is a promising approach to understand the prevalence of viruses in a given catchment population, as wastewater contains viruses from symptomatic and asymptomatic individuals. The current study reports the first detection of SARS-CoV-2 RNA in untreated wastewater in Slovenia. Two sizes of centrifugal filters were tested: 30 kDa and 10 kDA AMICON® Ultra-15 Centrifugal Filters, where 10 kDA resulted in a higher concentration factor and higher recovery efficiency. The results in hospital wastewater show that WBE can be used for monitoring COVID -19 and could be applied in municipal wastewater treatment plants as a potential complementary tool for public health monitoring at population level.
先前针对严重急性呼吸系统综合征冠状病毒（SARS-CoV）和中东呼吸综合征冠状病毒（MERS-CoV）的研究报告称，在有症状和无症状个体的粪便中均可检测到病毒 RNA。这些临床观察结果提示，受影响社区的市政和医院废水可能含有 SARS-CoV-2 RNA。最近的研究也报告了在人类粪便中存在 SARS-CoV-2 RNA。基于废水的流行病学（WBE）是一种很有前途的方法，可以了解特定集水区人群中病毒的流行情况，因为废水中包含有症状和无症状个体的病毒。本研究报告了首次在斯洛文尼亚未经处理的废水中检测到 SARS-CoV-2 RNA。研究测试了两种不同截留分子量的离心过滤器：30 kDa 和 10 kDa AMICON® Ultra-15 离心过滤器，其中 10 kDa 的截留分子量得到了更高的浓缩倍数和更高的回收效率。医院废水中的结果表明，WBE 可用于监测 COVID-19，并且可以在城市污水处理厂中应用，作为在人群水平上进行公共卫生监测的潜在补充工具。
