Instituto de Zoonosis Luis Pasteur, Av. Diaz Velez 4821 (1405), Ciudad Autónoma de Buenos Aires, Argentina.
Instituto de Zoonosis Luis Pasteur, Av. Diaz Velez 4821 (1405), Ciudad Autónoma de Buenos Aires, Argentina.
Rev Argent Microbiol. 2021 Apr-Jun;53(2):98-103. doi: 10.1016/j.ram.2020.06.012. Epub 2020 Nov 8.
The aim of this study was to estimate the diversity and prevalence of both groups of Brucella canis 1 and 2 with and without deletion respectively in different areas of Argentina. A total of 104 bacterial cultures were typed as B. canis strains using the classical biotyping method. Two PCR assays were performed to confirm that all isolates were B. canis and not Brucella suis. The differentiation between groups 1 and 2 was achieved using another PCR assay and the diversity of B. canis isolates was assessed with four MLVA_16 markers. All strains belonged to Group 2. Bruce 09 marker (MLVA_16 assay) showed the greatest diversity. Only Group 2 of B. canis was identified among the strains evaluated. The markers chosen from the MLVA_16 allowed us to detect genetic diversity among the strains of B. canis studied.
本研究旨在评估阿根廷不同地区的 Brucella canis 1 和 2 组及其缺失株的多样性和流行率。共对 104 株细菌培养物进行了经典生物分型，将其鉴定为 Brucella canis 株。采用两种 PCR 检测方法确认所有分离株均为 Brucella canis，而非 Brucella suis。通过另一种 PCR 检测方法区分 1 组和 2 组，使用 4 个 MLVA_16 标记评估 Brucella canis 分离株的多样性。所有菌株均属于第 2 组。Bruce 09 标记（MLVA_16 检测）显示出最大的多样性。在所评估的菌株中仅鉴定出 Brucella canis 的第 2 组。从 MLVA_16 中选择的标记允许我们检测研究中 Brucella canis 菌株的遗传多样性。
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