Yu Kaili, Xiong Shijiao, Xu Gang, Ye Xinhai, Yao Hongwei, Wang Fang, Fang Qi, Song Qisheng, Ye Gongyin
State Key Laboratory of Rice Biology and Key Laboratory of Agricultural Entomology of Ministry of Agriculture, Institute of Insect Sciences, Zhejiang University, Hangzhou, China.
College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, China.
Front Physiol. 2020 Oct 16;11:575655. doi: 10.3389/fphys.2020.575655. eCollection 2020.
Neuropeptides are a group of signal molecules that regulate many physiological and behavioral processes by binding to corresponding receptors, most of which are G-protein-coupled receptors (GPCRs). Using bioinformatic methods, we screened genomic and transcriptomic data of the ectoparasitoid wasp, , and annotated 34 neuropeptide candidate precursor genes and 44 neuropeptide receptor candidate genes. The candidate neuropeptide genes were found to encode all known insect neuropeptides except allatotropin, neuropeptide F, pigment dispersing factor, and CCHamides. When compared with the endoparasitic wasp and the ectoparasitic wasp , trissin and FMRFamide were found only in . A similar result held for the neuropeptide receptor genes, for the receptors were found in except the receptors of CCHamides and neuroparsin. Furthermore, we compared and analyzed the differences in neuropeptides in eight Braconidae wasps and identified natalisin in and , but not in the other wasps. We also analyzed the transcriptome data and qRT-PCR data from different developmental stages and tissues to reveal the expression patterns of the neuropeptides and their receptors. In this study, we revealed composition of neuropeptides and neuropeptide receptors in , which may contribute to future neurobiological studies.
神经肽是一类信号分子，它们通过与相应受体结合来调节许多生理和行为过程，其中大多数受体是G蛋白偶联受体（GPCRs）。我们使用生物信息学方法筛选了外寄生蜂的基因组和转录组数据，并注释了34个神经肽候选前体基因和44个神经肽受体候选基因。发现候选神经肽基因编码除促咽侧体素、神经肽F、色素分散因子和CCH酰胺外的所有已知昆虫神经肽。与内寄生蜂和外寄生蜂相比，仅在中发现了trissin和FMRF酰胺。神经肽受体基因也有类似结果，因为除了CCH酰胺和神经垂体素的受体外，在中发现了其他受体。此外，我们比较和分析了八种茧蜂科黄蜂神经肽的差异，并在和中鉴定出了新生素，但在其他黄蜂中未发现。我们还分析了不同发育阶段和组织的转录组数据和qRT-PCR数据，以揭示神经肽及其受体的表达模式。在本研究中，我们揭示了中的神经肽和神经肽受体组成，这可能有助于未来的神经生物学研究。
