Collaborative Innovation Center for the Origin and Control of Emerging Infectious Diseases, College of Basic Medical Sciences, Shandong First Medical University, Tai'an, China.
Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Sino-German Cooperative Research Centre for Zoonosis of Animal Origin of Shandong Province, Shandong Agricultural University, Tai'an, China.
Front Immunol. 2020 Oct 15;11:572289. doi: 10.3389/fimmu.2020.572289. eCollection 2020.
High-mobility group box 2 () belongs to the HMG-box family that participates in a variety of biologic processes. Recent studies have suggested that HMGB2 plays an important role in the innate immunity of fish. Cherry Valley duck is the main duck bred for meat consumption in China, but there is limited research available on the impact of duck HMGB2 () in antiviral innate immunity. Here, genes were first cloned and analyzed from the spleen of Cherry Valley ducks. We show that duHMGB2 is widely distributed in most tissues of healthy ducks, and duHMGB2 was differentially expressed in three organs (the spleen, brain, and lung) of ducks during different viral infections. duHMGB2 is mainly expressed in the nucleus of duck embryo fibroblast (DEF) cells. However, duHMGB2 is released into the cytoplasm after viral infection. DuHMGB2 induced expression of several genes that regulate the immune response. Moreover, duHMGB2 activated and upregulatede transcription factor NF-κB promoter activity. We also used single gene manipulations (knockout or overexpression) to confirm that duHMGB2 can inhibit the replication of duck plague virus, duck Tembusu virus, and the novel duck reovirus in DEF cells. These data show that duHMGB2 can activate the antiviral innate immunity of the host. Thus, may be considered an immune adjuvant against infectious diseases in duck.
高迁移率族蛋白 B2（HMGB2）属于 HMG 盒家族，参与多种生物学过程。最近的研究表明，HMGB2 在鱼类的固有免疫中发挥重要作用。樱桃谷鸭是中国主要的肉用鸭品种，但关于鸭 HMGB2（）在抗病毒固有免疫中的作用的研究有限。本研究首次从樱桃谷鸭脾脏中克隆和分析了基因。结果表明，duHMGB2 在健康鸭的大多数组织中广泛表达，并且在三种不同病毒感染的器官（脾脏、大脑和肺）中差异表达。duHMGB2 主要在鸭胚成纤维细胞（DEF）细胞核中表达，但在病毒感染后会释放到细胞质中。DuHMGB2 诱导了一些调节免疫反应的基因表达。此外，duHMGB2 激活并上调了转录因子 NF-κB 启动子活性。我们还通过单基因操作（敲除或过表达）证实，duHMGB2 可抑制 DEF 细胞中鸭瘟病毒、鸭坦布苏病毒和新型鸭呼肠孤病毒的复制。这些数据表明，duHMGB2 可以激活宿主的抗病毒固有免疫。因此， 可能被视为鸭传染性疾病的免疫佐剂。
