Sino-German Cooperative Research Centre for Zoonosis of Animal Origin of Shandong Province, Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an City, 271018, Shandong Province, China.
Collaborative Innovation Center for the Origin and Control of Emerging Infectious Diseases, College of Basic Medical Sciences, Shandong First Medical University, Tai'an City 271000, Shandong Province, China.
Poult Sci. 2022 Jan;101(1):101560. doi: 10.1016/j.psj.2021.101560. Epub 2021 Oct 20.
Interferon regulatory factor 4 (IRF4) is a multifunctional transcription factor that plays an important regulatory role in the interferon (IFN) signaling. IRF4 participates in the process of antivirus, Th cell differentiation and B cell maturation by regulating the expression of IFN and some lymphokines. In this study, Cherry Valley duck IRF4 (duIRF4) was cloned and its cDNA was analyzed. Expression of duIRF4 in a wide variety of tissues and changes in duIRF4 expression due to viral infection also was detected by quantitative real-time PCR. The results show that duIRF4 contains 1,341 bp of ORF encoding a protein with 446 amino acids and contains 3 domains: DNA-binding domain (DBD), IRF-association domain (IAD) and nuclear localization signal (NLS). Quantitative real-time PCR analysis showed that duIRF4 was evenly expressed in all tissues examined, with the highest expression in the spleen, followed by the bursa of Fabricius, and lower in the skin and brain. In addition, expression of duIRF4 in the brain and spleen was significantly upregulated after being infected by duck plague virus, duck Tembusu virus, and novel duck reovirus. These data suggest that duIRF4 may be involved in innate immune response.
干扰素调节因子 4（IRF4）是一种多功能转录因子，在干扰素（IFN）信号中发挥重要的调节作用。IRF4 通过调节 IFN 和一些淋巴因子的表达，参与抗病毒、Th 细胞分化和 B 细胞成熟的过程。本研究克隆了樱桃谷鸭 IRF4（duIRF4），并对其 cDNA 进行了分析。通过实时定量 PCR 检测了 duIRF4 在多种组织中的表达以及病毒感染后 duIRF4 表达的变化。结果表明，duIRF4 含有 1341bp 的 ORF，编码一个含有 446 个氨基酸的蛋白质，含有 3 个结构域：DNA 结合域（DBD）、IRF 结合域（IAD）和核定位信号（NLS）。实时定量 PCR 分析表明，duIRF4 在所有检测组织中均均匀表达，脾脏中的表达最高，其次是法氏囊，皮肤和大脑中的表达较低。此外，脑和脾组织感染鸭瘟病毒、鸭坦布苏病毒和新型鸭呼肠孤病毒后，duIRF4 的表达显著上调。这些数据表明，duIRF4 可能参与固有免疫反应。
