Jiao P R, Wei L M, Song Y F, Cui J, Zhang S, Han F, Yuan R Y, Liao M
College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an, Shandong 271018, China.
Poult Sci. 2015 Jun;94(6):1170-6. doi: 10.3382/ps/pev082. Epub 2015 Apr 3.
Laboratory of genetics and physiology 2 (LGP2) is an important intracellular receptor that recognizes viral RNAs in innate immunity. In this study, a novel LGP2 cDNA was identified from the spleen of a Muscovy duck (Cairina moschata). The deduced amino acid sequence of Muscovy duck LGP2 (MDLGP2) consisted of 675 amino acid residues. The peptide contained two main structure domains: six important motifs, including a DExD/H box for RNA helicase activity in the RNA helicase region located at the N-terminal region, and two Zn2+-binding regions with an RNA-binding loop in the C-terminus regulatory domain (CTD). The MdLGP2 mRNA was ubiquitously expressed in the tested tissues, with high expression levels in glandular stomach, colon, ileum, crop, and caecum tissues, and low expression levels in the brain, skin, and heart. The mRNA expression of MdLGP2 was significantly upregulated in the brain, spleen, and lungs of ducks in the early stages of postinfection with H5N1 highly pathogenic avian influenza virus (HPAIV). These results suggested that MdLGP2 was involved in the early stages of antiviral innate immune response in ducks after infection with H5N1 HPAIV. However, whether it plays a positive or negative regulatory role in the host antiviral response requires further investigation.
遗传学与生理学实验室2（LGP2）是一种重要的细胞内受体，在天然免疫中识别病毒RNA。在本研究中，从番鸭（Cairina moschata）脾脏中鉴定出一种新型LGP2 cDNA。番鸭LGP2（MDLGP2）推导的氨基酸序列由675个氨基酸残基组成。该肽包含两个主要结构域：六个重要基序，包括位于N端区域的RNA解旋酶区域中具有RNA解旋酶活性的DExD/H框，以及C端调节域（CTD）中带有RNA结合环的两个Zn2+结合区域。MdLGP2 mRNA在测试组织中普遍表达，在腺胃、结肠小肠、嗉囊和盲肠组织中表达水平较高，在脑、皮肤和心脏中表达水平较低。在感染H5N1高致病性禽流感病毒（HPAIV）后的早期阶段，番鸭脑、脾脏和肺中MdLGP2的mRNA表达显著上调。这些结果表明，MdLGP2参与了番鸭感染H5N1 HPAIV后抗病毒天然免疫反应的早期阶段。然而，它在宿主抗病毒反应中发挥正向还是负向调节作用尚需进一步研究。
