Ghalyanchilangeroudi A, Najafi H, Fallah Mehrabadi M H, Ziafati Kafi Z, Sadri N, Hojabr Rajeoni A, Modiri A, Safari A, Hosseini H
Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran, and Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran (current address).
Iran J Vet Res. 2020 Summer;21(3):230-233.
Avian infectious bronchitis (IB) is an infectious viral disease of chickens. The effective protection of chickens against many different infectious bronchitis virus (IBV) variants is not achieved unless the circulating genotypes in the region are identified and the cross-protection of the potential of vaccines in use is assessed.
In a monitoring program of IBVs, a new genotype was identified in the north of Iran, 2019. This work was conducted to isolate and characterize this new IBV genotype.
Tracheal tissues were collected from chickens showing signs of respiratory involvement. Specimens were homogenized and inoculated to the allantoic fluid of embryonated specific pathogen-free (SPF) eggs. Infectious bronchitis virus was detected using real time-polymerase chain reaction (RT-PCR). The hypervariable region of the IBV gene was amplified for sequencing.
Positive samples were phylogenetically analyzed, and both positive isolates were clustered with Q1 IBV strains.
This is the first report of the Q1 outbreak in Iran. More investigations are needed to find the role of Q1 IBV in the respiratory disease complex of chickens.
禽传染性支气管炎（IB）是鸡的一种病毒性传染病。除非确定该地区流行的基因型并评估所用疫苗的交叉保护潜力，否则无法有效保护鸡免受多种不同传染性支气管炎病毒（IBV）变种的侵害。
在一项IBV监测计划中，2019年在伊朗北部发现了一种新的基因型。开展这项工作以分离并鉴定这种新的IBV基因型。
从表现出呼吸道感染症状的鸡采集气管组织。将样本匀浆并接种到无特定病原体（SPF）胚胎蛋的尿囊液中。使用实时聚合酶链反应（RT-PCR）检测传染性支气管炎病毒。扩增IBV基因的高变区进行测序。
对阳性样本进行系统发育分析，两个阳性分离株均与Q1 IBV毒株聚类。
这是伊朗首次报告Q1毒株爆发。需要进行更多调查以确定Q1 IBV在鸡呼吸道疾病复合体中的作用。
