Najafi Hamideh, Langeroudi Arash Ghalyanchi, Hashemzadeh Masoud, Karimi Vahid, Madadgar Omid, Ghafouri Seyed Ali, Maghsoudlo Hossein, Farahani Reza Khaltabadi
Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, IRAN.
Department of Research and Production of Poultry Viral Vaccine, Razi Vaccine and Serum Research Institute, Karaj, IRAN.
Arch Virol. 2016 Jan;161(1):53-62. doi: 10.1007/s00705-015-2636-3. Epub 2015 Oct 14.
Infectious bronchitis (IB) is a viral avian disease with economic importance in the world, including Iran. S1 gene sequencing has been used for molecular epidemiological studies and genotypic characterization of infectious bronchitis virus (IBV). A total of 118 IBV isolates were obtained from tissue samples from chickens with clinically suspected IB from Iranian broiler farms (eight provinces, 200 samples). The isolates were confirmed by real-time polymerase chain reaction (PCR) and characterized by sequencing the spike glycoprotein gene. The isolates formed six distinct phylogenetic groups (IS/1494/06 [Var2] like, 4/91-like, IS/720-like, QX-like, IR-1 and Mass-like) that were related to variants isolated in the region. The most frequently detected viruses were of the Var2-like (IS/1494/06-like) genotype, with an overall prevalence of 34 %. Twenty-one percent of the isolates formed a cluster together with the 4/91 IBV type, 10 % were of the QX genotype, and 8 % were of the IS/720 genotype. In addition, 4 % and 3 % of the isolates belonged to the Massachusetts and IR-1 genotype, respectively. For the first time, we have isolated and characterized IBV variants from broiler farms in different provinces of Iran. This study demonstrates a constant evolution of IBV in Iran, demonstrating the need for continuous monitoring and development of new vaccines based on indigenous viruses.
传染性支气管炎（IB）是一种在包括伊朗在内的世界范围内具有经济重要性的禽类病毒性疾病。S1基因测序已用于传染性支气管炎病毒（IBV）的分子流行病学研究和基因型特征分析。从伊朗肉鸡养殖场（八个省份，200个样本）临床疑似患有IB的鸡的组织样本中总共获得了118株IBV分离株。通过实时聚合酶链反应（PCR）对分离株进行确认，并通过对刺突糖蛋白基因进行测序来进行特征分析。这些分离株形成了六个不同的系统发育组（IS/1494/06 [Var2]样、4/91样、IS/720样、QX样、IR-1和Mass样），它们与该地区分离出的变异株相关。最常检测到的病毒是Var2样（IS/1494/06样）基因型，总体流行率为34%。21%的分离株与4/91型IBV形成一个簇，10%为QX基因型，8%为IS/720基因型。此外，分别有4%和3%的分离株属于马萨诸塞州和IR-1基因型。我们首次从伊朗不同省份的肉鸡养殖场分离并鉴定了IBV变异株。这项研究表明IBV在伊朗不断进化，表明需要持续监测并研发基于本土病毒的新型疫苗。
