Ding Lan, Jiang Weifan, Chen Zhijun, Zhang Caiping, Tian Ying, Long Shiyin
Clin Lab. 2020 Nov 1;66(11). doi: 10.7754/Clin.Lab.2020.200241.
Inhibition of plasma cholesteryl ester transfer protein (CETP) can effectively reduce the risk of ath-erosclerotic cardiovascular disease by increasing high-density lipoprotein cholesterol (HDL-C) levels, but the effect of CETP on the distributions of HDL subclasses in patients with coronary heart disease (CHD) is still elusive.
To investigate the correlation between the level of CETP and the distributions of HDL subclasses, 121 healthy controls and 139 patients with CHD were selected as study subjects. The plasma levels of CETP and each HDL subclass were respectively determined by enzyme-linked immunosorbent assay and two-dimensional gel electrophoresis associated with the immunodetection method. At the same time, blood biochemical data from all subjects were collected, including the levels of triglycerides (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), HDL-C, apoA1, and apoB100. Correlation analysis and multiple regression analysis among the plasma HDL subclass values and biochemical parameters in subjects with CHD were conducted.
As the plasma level of CETP increases, the contents of TC, TG, and apoB100/A1 were obviously elevated, while the levels of HDL-C and apoA1 decreased significantly. For distributions of HDL subclasses, large-sized HDL2a and HDL2b were markedly decreased in the middle CETP group (p < 0.05) and the high CETP group (p < 0.001) compared to the low CETP group, while the small-sized preβ1-HDL was obviously increased. Intriguingly, when the plasma concentration of TC or TG in patients with CHD was higher, the elevated preβ1-HDL and reduced HDL2a were more dependent on the increase in CETP. Furthermore, correlation analysis and multiple regression analysis also confirmed that plasma CETP was positively correlated with preβ1-HDL levels and negatively correlated with HDL2b levels.
The distributions of HDL subclasses were associated with CETP in patients with CHD, especially in those with high levels of TC and TG. CETP levels were associated with an increase in small-sized preβ1-HDL and a decrease in large-sized HDL subclasses, which indicated that CETP might be a limiter of reverse cholesterol transport and HDL maturation.
抑制血浆胆固醇酯转运蛋白（CETP）可通过提高高密度脂蛋白胆固醇（HDL-C）水平有效降低动脉粥样硬化性心血管疾病的风险，但CETP对冠心病（CHD）患者HDL亚类分布的影响仍不明确。
为研究CETP水平与HDL亚类分布之间的相关性，选取121名健康对照者和139名CHD患者作为研究对象。分别采用酶联免疫吸附测定法和二维凝胶电泳结合免疫检测法测定血浆CETP水平和各HDL亚类水平。同时，收集所有受试者的血液生化数据，包括甘油三酯（TG）、总胆固醇（TC）、低密度脂蛋白胆固醇（LDL-C）、HDL-C、载脂蛋白A1（apoA1）和载脂蛋白B100（apoB100）水平。对CHD患者血浆HDL亚类值与生化参数进行相关性分析和多元回归分析。
随着血浆CETP水平升高，TC、TG和apoB100/A1含量明显升高，而HDL-C和apoA1水平显著降低。对于HDL亚类分布，与低CETP组相比，中CETP组（p < 0.05）和高CETP组（p < 0.001）中大型HDL2a和HDL2b明显减少，而小型前β1-HDL明显增加。有趣的是，CHD患者血浆TC或TG水平较高时，前β1-HDL升高和HDL2a降低更依赖于CETP的增加。此外，相关性分析和多元回归分析也证实，血浆CETP与前β1-HDL水平呈正相关，与HDL2b水平呈负相关。
CHD患者HDL亚类分布与CETP有关，尤其是在TC和TG水平较高的患者中。CETP水平与小型前β1-HDL增加和大型HDL亚类减少有关，这表明CETP可能是胆固醇逆向转运和HDL成熟的限制因素。
