Fernández-Segura E, Garcia J M, Campos A
Department of Cell Biology, Faculty of Medicine, University of Granada, Spain.
Histochemistry. 1990;94(3):315-21. doi: 10.1007/BF00266634.
The distribution patterns of acid phosphatase hydrolytic activity were studied in human peripheral blood cells with enzymocytochemical techniques together with light and scanning electron microscopy in the secondary and backscattered electron imaging modes. The acid phosphatase reaction product was seen in three different patterns of distribution: focal, granular and diffuse. These patterns were correlated with similar findings obtained with light microscopy. Acid phosphatase distribution patterns seen with SEM in the BEI mode were also correlated with the surface morphology of peripheral blood cells seen in the SEI mode. Cells exhibiting the focal pattern were smooth-surfaced with few microvilli; cells showing a granular pattern presented microvilli and microridges; ruffles were characteristic of cells with a diffuse pattern of activity. No reaction product was seen in cells bearing microvilli or ridges. Our findings demonstrate the correlation between acid phosphatase activity patterns and surface features in different subpopulations of peripheral blood cells.
采用酶细胞化学技术，结合光学显微镜以及二次电子成像和背散射电子成像模式的扫描电子显微镜，研究了人外周血细胞中酸性磷酸酶水解活性的分布模式。酸性磷酸酶反应产物呈现出三种不同的分布模式：局灶性、颗粒状和弥漫性。这些模式与光学显微镜下获得的类似结果相关。在背散射电子成像模式下用扫描电子显微镜观察到的酸性磷酸酶分布模式也与二次电子成像模式下观察到的外周血细胞表面形态相关。呈现局灶性模式的细胞表面光滑，微绒毛较少；呈现颗粒状模式的细胞有微绒毛和微嵴；有弥漫性活性模式的细胞以褶皱为特征。在有微绒毛或嵴的细胞中未观察到反应产物。我们的研究结果表明了外周血细胞不同亚群中酸性磷酸酶活性模式与表面特征之间的相关性。
