School of BioSciences, University of Melbourne, Parkville, Australia.
School of Mathematics and Statistics, University of Melbourne, Parkville, Australia.
Phys Biol. 2021 Jan 7;18(1):011002. doi: 10.1088/1478-3975/abc9d1.
Cell fate decision-making events involve the interplay of many molecular processes, ranging from signal transduction to genetic regulation, as well as a set of molecular and physiological feedback loops. Each aspect offers a rich field of investigation in its own right, but to understand the whole process, even in simple terms, we need to consider them together. Here we attempt to characterise this process by focussing on the roles of noise during cell fate decisions. We use a range of recent results to develop a view of the sequence of events by which a cell progresses from a pluripotent or multipotent to a differentiated state: chromatin organisation, transcription factor stoichiometry, and cellular signalling all change during this progression, and all shape cellular variability, which becomes maximal at the transition state.
细胞命运决策事件涉及许多分子过程的相互作用，从信号转导到遗传调控，以及一系列分子和生理反馈回路。每个方面本身都提供了一个丰富的研究领域，但为了理解整个过程，即使是简单的术语，我们也需要将它们放在一起考虑。在这里，我们试图通过关注细胞命运决策过程中的噪声作用来描述这个过程。我们使用一系列最近的结果来发展一个细胞从多能性或多能性到分化状态的事件序列的观点：染色质组织、转录因子计量和细胞信号都在这个过程中发生变化，并且都塑造了细胞的变异性，在过渡状态下达到最大值。
