College of Traditional Chinese Medicine, Changchun University of Chinese Medicine, Changchun 130117, China.
College of Traditional Chinese Medicine, Changchun University of Chinese Medicine, Changchun 130117, China.
Int Immunopharmacol. 2020 Nov;88:107017. doi: 10.1016/j.intimp.2020.107017. Epub 2020 Sep 22.
Pedunculoside (PE) is derived from the bark of iron holly, a member of the holly family. Previous studies have shown that PE has anti-inflammatory, antitumor, antiviral, cholesterol-lowering and blood-pressure-lowering effects. In this study, we aimed to investigate the effects of PE on ulcerative colitis and to explore its potential mechanisms. We treated a mouse model of ulcerative colitis induced by DSS (dextran sulfate sodium) with PE. The results showed that PE had an obvious effect on DSS-induced ulcerative colitis. PE significantly improved the colon length and clinical score in mice, and significantly inhibited the production of inflammatory cytokines. In the LPS-induced inflammatory response of RAW264.7 macrophages, we also found that PE significantly inhibited the phosphorylation of AKT, ERK1/2, JNK1/2, P65, and P38 to reduce the production of IL-1β, IL-6, TNF-α, COX-2, and iNOS. Furthermore, PE suppressed the LPS-induced transcriptional activities of nuclear factor P65 as well as the phosphorylation of P65. In addition, we also studied the effect of PE on LPS induced AKT/NF-κB and MAPK signaling pathways with primary peritoneal macrophages. In summary, PE has a beneficial effect on ulcerative colitis, and may be a potential natural product in the treatment of ulcerative colitis.
美登木素（PE）来源于冬青科的一种植物，苦丁茶。先前的研究表明，PE 具有抗炎、抗肿瘤、抗病毒、降胆固醇和降血压的作用。在这项研究中，我们旨在研究 PE 对溃疡性结肠炎的作用，并探讨其潜在的机制。我们用 PE 治疗由 DSS（葡聚糖硫酸钠）诱导的溃疡性结肠炎小鼠模型。结果表明，PE 对 DSS 诱导的溃疡性结肠炎有明显的作用。PE 显著改善了小鼠的结肠长度和临床评分，并显著抑制了炎症细胞因子的产生。在 LPS 诱导的 RAW264.7 巨噬细胞炎症反应中，我们还发现 PE 显著抑制了 AKT、ERK1/2、JNK1/2、P65 和 P38 的磷酸化，从而减少了 IL-1β、IL-6、TNF-α、COX-2 和 iNOS 的产生。此外，PE 抑制了 LPS 诱导的核因子 P65 的转录活性以及 P65 的磷酸化。另外，我们还研究了 PE 对 LPS 诱导的 AKT/NF-κB 和 MAPK 信号通路的影响，所用细胞为原代腹腔巨噬细胞。综上所述，PE 对溃疡性结肠炎有有益的作用，可能是治疗溃疡性结肠炎的一种有潜力的天然产物。
